The Resonance Frequency: Effects of Low Intensity Ultrasound on Attached and Floating Cells Viability

Proceedings of the 2nd International Conference on Biomedical Signal and Image Processing Pub Date : 2017-08-23 DOI:10.1145/3133793.3133809

Mariantonietta Ivone, L. Lamberti, C. Pappalettere

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Abstract

Cancer cell morphological, geometric and mechanic characterization could be important in relationship to therapy modality used. The objective of this work was to analyze the correlation between cell size and low intensity ultrasound sonication frequency for attached and floating cells. MCF7 and U937 cells were stressed by ultrasound in a range between 400 kHz and 620 kHz at 10 Hz PRF (Pulse Repetition Frequency) for three minutes. Immediately after sonication, the vitality and the diameter were evaluated. Although sensitivity to frequency is more evident for attached cells, some effect can be clearly detected also for floating cells: it seems that each fixed frequency prefers to kill floating cell with a particular value of diameter, with a maximum value of killing rate. The results obtained in this research work support the frequency plays a significant role on cell treatment. This result could be interesting not only to explain the proven ultrasound selectivity but also to destroy cancer cells, preserving healthy cells.

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共振频率:低强度超声对附着和漂浮细胞活力的影响

癌细胞的形态、几何和力学特征可能与所使用的治疗方式有关。本研究的目的是分析附着细胞和漂浮细胞的低强度超声频率与细胞大小的关系。MCF7和U937细胞在400 kHz和620 kHz范围内以10 Hz PRF(脉冲重复频率)进行超声应激3分钟。超声后，立即评估活力和直径。虽然附着细胞对频率的敏感性更明显，但对于浮动细胞也可以清楚地检测到一些影响:似乎每个固定频率更倾向于杀死具有特定直径值的浮动细胞，其杀死率最大值。本研究结果支持该频率在细胞治疗中起重要作用。这个结果可能很有趣，不仅可以解释已证实的超声波选择性，还可以摧毁癌细胞，保留健康细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Proceedings of the 2nd International Conference on Biomedical Signal and Image Processing

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