Study of physico-chemical factors affecting the growth of cell-culture adapted bovine Rotavirus strain of Pakistan

Abstract

Rotavirus (RV) diarrhea is the major cause of death of millions of children in developing countries besides causing economically significant malady in neonates of many domestic animals. There is a very little information available for the factors which can affect prevalence of Bovine Rotavirus (BRV) in Pakistan. There is a dire need to propagate BRV on cell culture and evaluate the physical and chemical control of RV in effective ways for the betterment of human and livestock welfare. Therefore, the present study aimed to propagate BRV on Madin-Darby bovine kidney (MDBK) cell line. The screening of the virus was done by using commercially available kit and TCID50 technique. The propagation of BRV was then led to study its inactivity and infectivity potential using different physical and chemical factors. For this reason, 3 physical factors (Temperature, pH and UV light) and 8 chemical disinfectants were used. The virus got completely inactive at a temperature range of 75 to 80oC and 5.00 to 6.00 pH while remained active at 7.04 and 8.00. The virus was inactivated after exposure to 0.5% Virkon®-S within 30 minutes, 0.5% Bromo-Sept after 30 min, and 1.0% surf excel after 30 min Phenol within 60 min at a concentration of 1.0% and 0.6% H2O2 after 30 min proved to be virucidal for the virus. These findings can be helpful for the farmers to keep their farms decontaminated from this virus. Moreover, these results can also be of help in the prevention of the outbreaks that occur in the hospitals. #These authors contributed equally to this work.