[Optimized determination and properties of NADPH-dependent glutathione reductase in serum. Studies on serum glutathione reductase, I. (author's transl)].

G Weidemann
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Abstract

Reaction conditions were optimized for the determination of serum glutathione reductase, which has not yet been investigated systematically. Imidazole was found to be the most suitable buffer material; the highest glutathione reductase activity in serum was always obtained with imidazole/HCl buffer, which, in contrast to all other tested buffers, also resulted in the maximal enzyme activity without preincubation. In imidazole buffer, the pH-activity curve of serum glutathione reductase shows a broad optimum between pH 6.5 and 6.9. A GSSG concentration of 2 mmol/l and a NADPH concentration of 0.43 mmol/l gave maximal enzyme activity and a linear reaction over 10 min up to 20 U/l test solution. An investigation of serum glutathione reductase activity from 100 clinically healthy probands gave values between 20 and 50 U/l. In the optimized assay system the glutathione reductase in the serum reacts specifically with GSSG and NADPH.

优化血清中nadph依赖性谷胱甘肽还原酶的测定及性质。血清谷胱甘肽还原酶的研究[j]。
对测定血清谷胱甘肽还原酶的反应条件进行了优化,但尚未进行系统的研究。咪唑是最合适的缓冲材料;血清中谷胱甘肽还原酶活性最高的总是咪唑/盐酸缓冲液,与所有其他测试的缓冲液相比,该缓冲液在没有预孵育的情况下也产生了最大的酶活性。在咪唑缓冲液中,血清谷胱甘肽还原酶的pH-活性曲线在pH 6.5 ~ 6.9范围内呈现广泛的最优。当GSSG浓度为2 mmol/l, NADPH浓度为0.43 mmol/l时,酶活性最高,在20 U/l的测试溶液中,酶活性在10 min内呈线性反应。对100名临床健康先证者血清谷胱甘肽还原酶活性的调查给出了20至50 U/l的值。在优化的检测系统中,血清中的谷胱甘肽还原酶与GSSG和NADPH有特异性反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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