Application of three dimensional image analysis to the mammalian cell nucleus

Abstract

A three dimensional image segmentation and analysis system is proposed to study the confocal microscopy images of DNA replication sites in cell nuclei. The algorithm presented here includes detection of replication sites in 2D slices in the image and reconstructing 3D objects from 2D data. We also present a user interface to allow easy interaction with both the segmentation algorithm and the 3D segmented data.<>