Thin-Film Transistor Platform for Electrophysiological and Electrochemical Characterization of Biological Cells

Abstract

This paper presents a locally-addressable 2-dimensional arrayed transparent electrode platform with integrated Thin-Film Transistor (TFT) for electrophysiology. 2D electrical measurements on 28 parallel-connected lines selected from a 22,500 microelectrode array were successfully performed for heart cell investigation for the first time. Amperometry measurement was also demonstrated using transparent electrodes functionalized with Ag/AgCl by additive manufacturing. The applicability of this transparent TFT substrate platform to electrochemical sensors was confirmed by experiments. The platform offers a unique access to versatile lab-on-a-chip devices that integrate many measurement techniques on one chip (electrical, chemical and optical) for the study of large cell cultures, tissues or organoids.