Minu Singh, D. Kumari, Pooja Chahar, Yogita Singhal, Radhika Singh
{"title":"Effect of different microbial culture on biohydrogen production from Benincasa hispida waste","authors":"Minu Singh, D. Kumari, Pooja Chahar, Yogita Singhal, Radhika Singh","doi":"10.1109/R10-HTC.2016.7906803","DOIUrl":null,"url":null,"abstract":"Biohydrogen production has gained attention in recent years using anaerobic degradation method. In the present study, Benincasa hispida (petha) solid waste generated from industries were used as substrate. Three different inoculum namely, cow dung (CD), goat dung (GD) and pretreated soil (SP) were used as a bacterial source. They were used separately and also in combination of two cultures in equal ratio. The cultures then mixed with petha waste slurry in 20:80 in batch reactor of 500 mL with a working volume of 400 mL under anaerobic conditions. Substrate used in the present study had chemical oxygen demand and glucose concentration of 3150 mg/L and 11 mg/L respectively. Biohydrogen was found to be maximum in GD+CD (87%) used as the bacterial inoculum. Acetic acid was also estimated and a maximum concentration of 2650 ppm was found in the reactor operated using GD as bacterial source. Degradation in various parameters was also studied in terms of COD, glucose.","PeriodicalId":174678,"journal":{"name":"2016 IEEE Region 10 Humanitarian Technology Conference (R10-HTC)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2016 IEEE Region 10 Humanitarian Technology Conference (R10-HTC)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/R10-HTC.2016.7906803","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Biohydrogen production has gained attention in recent years using anaerobic degradation method. In the present study, Benincasa hispida (petha) solid waste generated from industries were used as substrate. Three different inoculum namely, cow dung (CD), goat dung (GD) and pretreated soil (SP) were used as a bacterial source. They were used separately and also in combination of two cultures in equal ratio. The cultures then mixed with petha waste slurry in 20:80 in batch reactor of 500 mL with a working volume of 400 mL under anaerobic conditions. Substrate used in the present study had chemical oxygen demand and glucose concentration of 3150 mg/L and 11 mg/L respectively. Biohydrogen was found to be maximum in GD+CD (87%) used as the bacterial inoculum. Acetic acid was also estimated and a maximum concentration of 2650 ppm was found in the reactor operated using GD as bacterial source. Degradation in various parameters was also studied in terms of COD, glucose.