Detection And Antibiotic Susceptibility Test of Enterococcus Spp. from Different Clinical Samples in Duhok City/ Iraq

Rand Bahdeen Jalal, Bland Husamuldeen Abdullah
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Abstract

Enterococci a variety of species and formerly believed to be of no medical importance; recently they emerged as life threatening multidrug-resistant (MDR) nosocomial pathogens. Enterococcus faecalis and Enterococcus faecium make up the majority of clinical isolates. Furthermore, these bacteria are naturally resistant to most commonly used antibiotics. The study assesses the prevalence of Enterococcus spp. in different clinical samples; pinpoints the prevalence of species causing infection in this area and finally analyzes the patterns of drug resistance of these isolates. A total of 560 samples were collected and plated on blood and chocolate agar. Species identification and antibiotic susceptibility testing were performed using automated VITEK 2 system. Four species of Enterococci were isolated: E. faecalis (74%), E. faecium (18%) and only (4%) of each E. gallinarum and E. avium. E. faecalis had a (100%) rate of resistance to Gentamycin. E. faecium , a rate of (100%) resistance to Benzylpenicillin. E. gallinarum showed complete resistance to a variety of antibiotics. E. avium showed complete resistance to Ampicillin and Tetracycline. Enterococci represent an infection control challenge in the region, a careful usage of antibiotics and implementation of infection control practices is believed to reduce the risk of infection.
伊拉克杜霍克市不同临床标本肠球菌的检测及药敏试验
肠球菌:各种各样的肠球菌,以前被认为没有医学意义;最近它们成为威胁生命的耐多药(MDR)院内病原体。粪肠球菌和粪肠球菌占临床分离菌的大多数。此外,这些细菌对大多数常用抗生素具有天然耐药性。本研究评估了肠球菌在不同临床样本中的患病率;查明该地区引起感染的物种的流行情况,最后分析这些分离株的耐药模式。共收集了560个样本,并将其镀于血液和巧克力琼脂上。采用自动VITEK 2系统进行菌种鉴定和药敏试验。分离出4种肠球菌:粪肠球菌占74%,粪肠球菌占18%,鸡肠球菌和鸟肠球菌各占4%。粪肠球菌对庆大霉素的耐药率为100%。粪肠杆菌对青霉素的耐药率(100%)。鸡大肠杆菌对多种抗生素表现出完全的耐药性。禽大肠杆菌对氨苄西林和四环素完全耐药。肠球菌对该地区的感染控制构成挑战,谨慎使用抗生素和实施感染控制措施被认为可降低感染风险。
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