EFFECTS OF DIFFERENT NATURAL IRRIGANT SOLUTIONS ON SURFACE ROUGHNESS OF ROOT CANAL DENTIN USING QUANTITATIVE TOPOGRAPHICAL 3D SURFACE TEXTURE ANALYSIS

Hemat M. Elsheikh, Heba Abdelkafy, Inas M. Al-Sherbiny, R. Marzouk, Seham Hallool
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Abstract

Objective: was to investigate the effect of 0.2% chitosan, 0.2% nano chitosan, and 5% apple vinegar compared to 17% EDTA on intraradicular dentin surface roughness. Materials and Methods: Twenty single-rooted human teeth were used in this study, after decoronation, working length determination, and mechanical preparation using ProTaper Next rotary files with irrigation after each file using 5ml of 2.6% NaOCl. The specimens were randomly divided into four groups according to the final irrigating solution. Group I used 17%EDTA, group II with 0.2% chitosan, group III with 0.2% nano-chitosan, and group IV with 5% apple vinegar. Specimens were sectioned longitudinally and evaluated for surface roughness changes using Environmental Scanning Electron Microscope (ESEM) and a photomicrograph was analyzed using a 3D software system. Results: The highest mean value of surface roughness was recorded in group IV treated with 5% apple vinegar with a statistically significant difference from the other groups (P value< 0.05). The lowest mean value was observed in group III used 0.2% nano-chitosan followed by group II irrigated with 0.2% chitosan which was lower than the control group I used 17% EDTA with statistically significant difference between the three groups (P value< 0.05). Conclusion: 0.2% nano-chitosan as the final irrigating solution had minimal drawbacks on dentin micro-structures by producing a slight change of surface roughness .
不同天然灌溉溶液对根管牙本质表面粗糙度的影响,采用定量地形三维表面纹理分析
目的:研究0.2%壳聚糖、0.2%纳米壳聚糖和5%苹果醋与17% EDTA对根内牙本质表面粗糙度的影响。材料与方法:本研究选用20颗单根人牙,用ProTaper Next旋转锉进行装饰、测定工作长度、机械制备,每锉后用5ml 2.6% NaOCl冲洗。根据最终的冲洗溶液将标本随机分为四组。1组为17%EDTA, 2组为0.2%壳聚糖,3组为0.2%纳米壳聚糖,4组为5%苹果醋。对样品进行纵向切片,使用环境扫描电子显微镜(ESEM)评估表面粗糙度变化,并使用3D软件系统分析显微照片。结果:表面粗糙度平均值以5%苹果醋处理组最高,与其他组比较差异有统计学意义(P值< 0.05)。以0.2%纳米壳聚糖组最低,其次为0.2%壳聚糖组,低于17% EDTA组,三组间差异有统计学意义(P值< 0.05)。结论:以0.2%纳米壳聚糖作为最终冲洗液,牙本质表面粗糙度变化不大,对牙本质微结构的影响最小。
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