PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation

Journal of nutrition & intermediary metabolism Pub Date : 2017-12-01 DOI:10.1016/j.jnim.2017.11.003

Muni Swamy Ganjayi , Balaji Meriga , Balaji Hari , Lokanatha Oruganti , Sreenivasulu Dasari , Ramgopal Mopuri

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引用次数: 10

Abstract

The prevalence of obesity and associated ailments have shown tremendous rise in recent times and drew the attention of researchers to explore effective therapeutic alternatives. In the present study, polyphenolic rich fraction of Terminalia paniculata bark (TPPF) was evaluated for its ability to inhibit adipogenesis, pancreatic lipase and amylase activity. The maximum inhibitory activity of lipase (78%) and amylase (81%) was noticed at 250 μg/mL of TPPF. During cytotoxicity assay with MTT, TPPF did not show any cytotoxic effect on 3T3-L1 cells up to a concentration of 400 μg/mL. Oil Red O staining of 3T3-L1 cells showed considerable reduction in adipocytes differentiation and lipid accumulation in the presence of TPPF (250 μg/mL) when compared with untreated 3T3-L1 cells. HPLC analysis of TPPF revealed the presence of Gallic acid, Ellagic acid and Quercetin as the major components. Our results suggest that, TPPF may be useful in attenuating obesity ailments.

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细穗富多酚组分通过抑制胰淀粉酶、脂肪酶和3T3-L1脂肪细胞分化来减轻肥胖

近年来，肥胖和相关疾病的发病率急剧上升，这引起了研究人员的注意，他们正在探索有效的治疗方法。摘要本研究以黄芩树皮富多酚部位(TPPF)为研究对象，研究其对脂肪生成、胰脂肪酶和淀粉酶活性的抑制作用。250 μg/mL TPPF对脂肪酶和淀粉酶的抑制活性最高，分别为78%和81%。MTT细胞毒实验显示，当TPPF浓度达到400 μg/mL时，TPPF对3T3-L1细胞无细胞毒作用。3T3-L1细胞油红O染色显示，与未处理的3T3-L1细胞相比，在TPPF (250 μg/mL)存在下，脂肪细胞分化和脂质积累明显减少。高效液相色谱分析显示，其主要成分为没食子酸、鞣花酸和槲皮素。我们的研究结果表明，TPPF可能有助于减轻肥胖疾病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of nutrition & intermediary metabolism

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审稿时长

12 weeks