Point-of-care Parvovirus B19 detection and genotyping based on microfluidics and chemiluminescence “contact” imaging detection

Abstract

A diagnostic device suitable for Parvovirus B19 genotyping and quantitative DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications.