Suppression of opportunistic infectious bacteria by lactic acid bacteria and antibacterial drugs

Asian Pacific Journal of Dentistry Pub Date : 1900-01-01 DOI:10.47416/apjod.19-0255

T. Nakano, S. Imai, Takumi Ishikawa, Tomohiko Terai, T. Okumura, N. Hanada, H. Kawahara

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引用次数: 2

Abstract

Purpose: The objective of this study was to investigate inhibitory effect of lactic acid bacteria and antibiotics on the growth of Streptococcus pneumoniae ATCC 33400. Materials and Methods: Antibiotic activities of five oral probiotic candidate bacteria including two strains of Lactobacillus crispatus, Lactobacillus fermentum, Lactobacillus gasseri and Streptococcus mitis and several antibiotics against S. pneumoniae were investigated using radial diffusion assay and competition assay. The biofilm formation of S. pneumoniae in the presence of 50% saturated ammonium sulfate precipitation fraction from L. gasseri and several concentrations of gentamicin were monitored by real time cell analyzer, xCELLigence. Results: The growth of S. pneumoniae was inhibited by two strains of Lactobacillus crispatus, Lactobacillus fermentum and Lactobacillus gasseri. The saturated ammonium sulfate precipitation fraction (0-30% and 30-50%) from L. gasseri effectively inhibited growth of S. pneumoniae. The biofilm formation of S. pneumoniae monitored by real time cell analyzer (xCELLigence) was suppressed by gentamicin in dose dependent manner. Antibacterial substance from L. gasseri against S. pneumoniae was suggested to be small molecular weight substance. Conclusion: L. gasseri inhibited the growth of S. pneumoniae suggesting that L. gasseri will be a candidate of probiotics. (Asian Pac J Dent 2019; 19: 17-25.)

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乳酸菌与抗菌药物对机会感染性细菌的抑制作用

目的:探讨乳酸菌和抗生素对肺炎链球菌ATCC 33400生长的抑制作用。材料与方法:采用径向扩散法和竞争法研究了5种口服益生菌候选菌的抗菌活性，其中包括2株马铃薯乳杆菌、发酵乳杆菌、发酵乳杆菌和脓毒性链球菌，以及几种抗生素对肺炎链球菌的抗菌活性。利用实时细胞分析仪xCELLigence监测了50%饱和硫酸铵沉淀馏分和不同浓度庆大霉素对肺炎链球菌生物膜形成的影响。结果:两株发酵乳杆菌、发酵乳杆菌和发酵乳杆菌均能抑制肺炎链球菌的生长。饱和硫酸铵沉淀分数(0 ~ 30%和30 ~ 50%)对肺炎链球菌生长有明显抑制作用。庆大霉素对实时细胞分析仪(xCELLigence)监测的肺炎链球菌生物膜形成呈剂量依赖性抑制作用。结果表明，加氏乳杆菌对肺炎链球菌的抑菌物质为小分子量物质。结论:加氏乳杆菌能抑制肺炎链球菌的生长，提示加氏乳杆菌可能是益生菌的候选菌。(亚太杂志2019;19: 17-25。)

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Asian Pacific Journal of Dentistry

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