A Gradient Scaffold with Segregated Hydrophilicity-Hydrophobicity Enables Reconstruction of Goat's Hierarchical Temporomandibular Joint Condyle Cartilage
Xi Yu, Yihui Hu, L. Zou, Shifeng Yan, Huimin Zhu, Kunxi Zhang, W. Liuc, D. He, Jingbo Yin
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引用次数: 0
Abstract
Temporomandibular joint (TMJ) supports chewing, talking or other daily oral activities. So far, it still remains a great challenge to treat the defected TMJ condyle cartilage through tissue engineering technology. Herein, a bilayered gradient scaffold is designed to fully reconstruct the different cartilage matrices of TMJ condyle under same induction condition. The gradient scaffold with segregated hydrophobicity-hydrophilicity in top and bottom layer is prepared from a low and high content of polyethylene glycol (PEG) crosslinked poly (L-glutamic acid)- g -polycaprolactone (PLGA- g -PCL). The hydrophobic microdomains in top layer support the adhesion and spread of bone mesenchymal stem cells (BMSCs), thus inducing the differentation towards fibrocartilage; while aggregates (spheroids) are formed on the hydrophlic bottom layer, showing a preferable hyaline differentiation pathway under same chondrogenic induction in vitro . After 14 d in vitro induction, the scaffold/BMSCs construct is implanted in goat’s TMJ condyle defects. The post-operative outcome after 2 months demonstrates that the defects are fully covered by neo-cartilage , and the regenerated hierarchical TMJ condyle cartilage is perfectly consisted of ordered fibrocartilage and hyaline cartilage, which is same as natural condyle cartilage, corroborating that this gradient scaffold with segregated hydrophilicity-hydrophobicity carrying induced BMSCs is a promising for treatment of TMJ condyle cartilage defects.
颞下颌关节(TMJ)支持咀嚼、说话或其他日常口腔活动。目前,利用组织工程技术治疗TMJ髁突软骨缺损仍然是一个很大的挑战。本文设计了一种双层梯度支架,在相同诱导条件下完全重建TMJ髁的不同软骨基质。以低含量和高含量聚乙二醇(PEG)交联聚(l -谷氨酸)- g -聚己内酯(PLGA- g - pcl)为原料制备了上下两层亲疏分离的梯度支架。顶层疏水微域支持骨间充质干细胞(BMSCs)的粘附和扩散,从而诱导其向纤维软骨分化;而聚集体(球状体)在亲水底层形成,在体外相同的软骨诱导下表现出较好的透明分化途径。体外诱导14 d后,将支架/骨髓间充质干细胞构建物植入山羊TMJ髁突缺损。术后2个月的结果显示缺损被新生软骨完全覆盖,再生的分层状TMJ髁突软骨完全由有序的纤维软骨和透明软骨组成,与天然髁突软骨相同,证实了这种亲疏水分离携带诱导骨髓间充质干细胞的梯度支架是治疗TMJ髁突软骨缺损的一种很有前景的支架。