Asimple test for immunogenicity of colloidal infusion solutions- the draining lymph node activation.

Abstract

The increasing number of lymphocytes with nucleoli synthesizing RNA in the mouse lymph nodes, draining the site of injection of infusion solutions, was used as a marker for their immunogenicity. The percentage of "active" lymphocytes significantly increased 3 days after the administration of preparations based on bovine serum or human haemoglobin which were found immunogenic when testing for antibody formation. Such a reaction was not elicited in mice treated with Physiogel, Dextran and Duxon which do not cause any production of antibodies. The described test may serve as a rapid and economical assay for the immunogenicity of infusion solutions and other substances.