[Isolation of Ancylostoma duodenale antigens and production of immune sera].

Abstract

Rabbits were immunized using intact larvae or homogenates from Ancylostoma duodenale. Antisera were tested by immunodiffusion. The homogenates promote the formation of antibodies but the intact worms were not able to induce them. The antisera were partially purified by precipitation with amonium sulphate 40% saturation and filtration through Sephadex G-200. The purified material was attached to Sepharose 6B and used as immunoabsorbent for the isolation of the antigens from the soluble extracts of parasites. The isolated antigens were used in order to obtain new antisera. These antisera were used for the preparation of more efficient immunoabsorbent which allow to isolate new antigens that gave three precipitation lines by immunodiffusion. The polyacrylamide gel electrophoresis of crude homogenate discriminate 12 components, and the electrophoresis of the isolated antigens gave only 3 bands.