[Production of alkaline protease from acid cheese whey].

Abstract

The use of acid cheese whey as an essential substrate for the production of alkaline protease was studied by growing Bacillus subtilis NRRL 3411 on different conditions of aereation. The maximum enzymatic activity of 17 000 proteolytic units was obtained in the following conditions: 200 RPM, air flow of 1 v/v minutes (KLa 37 h-1), separate sterilization of the medium components and periodical additions of peptone.