{"title":"Immunogenicity of glutaraldehyde-treated homologous albumin in rabbits","authors":"Doina Onica˛, Rodica Lenkei, Victor Gheţie","doi":"10.1016/0161-5890(78)90043-3","DOIUrl":null,"url":null,"abstract":"<div><p>Glutaraldehyde treatment of rabbit albumin corresponding to the 8/1 albumin/glutaraldehyde weight ratio conferred to the molecule new immunogenic properties, by inducing two types of antigenic determinants. One of them was found on the monomeric fraction of glutaraldehyde-treated albumin, while the other on the polymeric one. A hypothesis about the possible distribution of the determinants on albumin polymers dependent on the glutaraldehyde concentration used for polymerization is presented. Glutaraldehyde treatment induced similar antigenic determinants on albumin of different origins as shown by the cross-reactivity between glutaraldehyde-treated human and rabbit albumin, which precipitate both with human and rabbit antibodies. Rabbit antibodies were shown to be specific for glutaraldehyde-polymerized albumin, as they failed to react with untreated albumin as well as albumin molecules treated by other means than glutaraldehyde (heat and acid treatment).</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 9","pages":"Pages 687-693"},"PeriodicalIF":0.0000,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90043-3","citationCount":"32","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078900433","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 32
Abstract
Glutaraldehyde treatment of rabbit albumin corresponding to the 8/1 albumin/glutaraldehyde weight ratio conferred to the molecule new immunogenic properties, by inducing two types of antigenic determinants. One of them was found on the monomeric fraction of glutaraldehyde-treated albumin, while the other on the polymeric one. A hypothesis about the possible distribution of the determinants on albumin polymers dependent on the glutaraldehyde concentration used for polymerization is presented. Glutaraldehyde treatment induced similar antigenic determinants on albumin of different origins as shown by the cross-reactivity between glutaraldehyde-treated human and rabbit albumin, which precipitate both with human and rabbit antibodies. Rabbit antibodies were shown to be specific for glutaraldehyde-polymerized albumin, as they failed to react with untreated albumin as well as albumin molecules treated by other means than glutaraldehyde (heat and acid treatment).
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