[Transfer electron and hydrogen atom in model and enzymatic reactions of NAD and NADP].

Abstract

The paper deals with the mechanism of some model reactions of NAD and NADP which proceed with transfer of one electron and hydrogen atom. The joint oxidation of N-benzyl-1,4-dihydronicotinamide (I) and phenylglyoxal with hydrogen peroxide in the presence of cupric ions is found to result in transfer of one hydrogen atom from dihydropyridine molecule to hydroxy-radical and the electron to a molecule of oxygen or dinitrobenzene. The oxydation of I with benzoquinone is found to proceed with formation of free radicals as intermediate. The mechanism of NAD reduction with sodium hydrosulphite is proposed.