Elimination of lipaemic interference by high-speed centrifugation.

IF 3.8 3区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Gemma Solé-Enrech, Ruth Cano-Corres, Maria Isabel Aparicio-Calvente, Nino Spataro
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引用次数: 0

Abstract

Introduction: In order to deliver high quality results, detection and elimination of possible analytical interferences, such as lipaemia, is crucial. The aim of this study is to evaluate the efficacy of high-speed centrifugation in eliminating lipaemic interference and to define own lipaemic index (LI) for the studied biochemical analytes.

Materials and methods: Evaluated analytes were: albumin, alkaline phosphatase, alanine-aminotransferase (ALT), aspartate-aminotransferase (AST), calcium, creatinine, gamma-glutamyltransferase (GGT), glucose, phosphates, total proteins, urea and total bilirubin. Those analytes and LIs have been analysed in duplicate in the Roche Diagnostics-c8000 analyser in samples centrifuged at 3000 rpm/10 minutes in the SL16 (Thermo Scientific, Waltham, USA) centrifuge and according to an own high-speed centrifugation protocol (12,900 rpm/15 minutes) in the MicroCL17R (Thermo Scientific, Waltham, USA) centrifuge. Lipaemia has been measured in each sample. The efficiency of high-speed centrifugation is verified by the Wilcoxon test (P < 0.05). In cases where significant differences are observed, our own LI is calculated. For ALT and AST, it is verified by McNemar test (P < 0.05). For creatinine, both Wilcoxon and McNemar test were applied.

Results: There were statistically significant differences in analyte concentration before and after high-speed centrifugation for: albumin, creatinine, GGT, glucose, phosphates, urea and total bilirrubin. Own LI is calculated. McNemar test shows statistically significant diferences in the proportion of delivered results before and after high-speed centrifugation in ALT, AST and creatinine.

Conclusions: This study confirms the efficacy of high-speed centrifugation protocol for all the considered analytes, excepting calcium, alkaline phosphatase and total proteins.

Abstract Image

高速离心消除血脂干扰。
为了提供高质量的结果,检测和消除可能的分析干扰,如血脂,是至关重要的。本研究的目的是评价高速离心对消除脂化干扰的效果,并为所研究的生化分析物定义自身的脂化指数(LI)。材料与方法:评价物为:白蛋白、碱性磷酸酶、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、钙、肌酐、γ -谷氨酰转移酶(GGT)、葡萄糖、磷酸盐、总蛋白、尿素和总胆红素。这些分析物和LIs在Roche Diagnostics-c8000分析仪中,在SL16 (Thermo Scientific, Waltham,美国)离心机中以3000 rpm/10分钟的速度离心,在MicroCL17R (Thermo Scientific, Waltham,美国)离心机中按照自己的高速离心方案(12,900 rpm/15分钟)进行分析。在每个样本中都测量了血脂。采用Wilcoxon检验验证高速离心的效率(P < 0.05)。在观察到显著差异的情况下,计算我们自己的LI。ALT和AST经McNemar检验证实(P < 0.05)。肌酐采用Wilcoxon试验和McNemar试验。结果:白蛋白、肌酐、GGT、葡萄糖、磷酸盐、尿素、总胆红素高速离心前后分析物浓度差异有统计学意义。计算自己的LI。McNemar检验显示高速离心前后ALT、AST、肌酐的送检比例差异有统计学意义。结论:本研究证实了高速离心方案对除钙、碱性磷酸酶和总蛋白外的所有考虑的分析物的有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biochemia Medica
Biochemia Medica 医学-医学实验技术
CiteScore
5.50
自引率
3.00%
发文量
70
审稿时长
>12 weeks
期刊介绍: Biochemia Medica is the official peer-reviewed journal of the Croatian Society of Medical Biochemistry and Laboratory Medicine. Journal provides a wide coverage of research in all aspects of clinical chemistry and laboratory medicine. Following categories fit into the scope of the Journal: general clinical chemistry, haematology and haemostasis, molecular diagnostics and endocrinology. Development, validation and verification of analytical techniques and methods applicable to clinical chemistry and laboratory medicine are welcome as well as studies dealing with laboratory organization, automation and quality control. Journal publishes on a regular basis educative preanalytical case reports (Preanalytical mysteries), articles dealing with applied biostatistics (Lessons in biostatistics) and research integrity (Research integrity corner).
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