Evaluation of lateral flow and ELISA techniques for detecting IgG and IgM antibodies in COVID-19 cases in Türkiye.

IF 1.7 4区医学 Q3 HEALTH CARE SCIENCES & SERVICES

Eastern Mediterranean Health Journal Pub Date : 2023-02-26 DOI:10.26719/emhj.23.011

Ayse Arikan, Osman Doluca, Sila Akhan, Tamer Sanlidag, Murat Sayan

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引用次数: 0

Abstract

Background: Antibody testing can complement molecular assays for detecting COVID-19.

Aims: We evaluated the concurrence between lateral flow assay and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

Methods: The study was conducted at Kocaeli University, Türkiye. We used a lateral flow assay and ELISA to test serum samples from COVID-19 cases, confirmed by polymerase chain reaction assays (study group) and pre-pandemic stored serum samples (control group). We used Deming regression to evaluate the antibody measurements.

Results: The study group included 100 COVID-19 cases, and the control group included pre-pandemic samples from 156 individuals. The lateral flow assay detected immunoglobulin M (IgM) and G (IgG) antibodies in 35 and 37 study group samples. ELISA detected IgM nucleocapsid (N) antibodies in 18 samples, and IgG (N) and IgG spike 1 (S1) antibodies in 31 and 29 samples, respectively. None of the techniques detected antibodies in the control samples. Strong correlations were found between lateral flow IgG (N+ receptor-binding domain + S1) and ELISA IgG (S) (r = 0.93, P < 0.01) and ELISA IgG (N) (r = 0.81, P < 0.01). Weaker correlations were seen between ELISA IgG S and IgG N (r = 0.79, P < 0.01) and lateral flow assay and ELISA IgM (N) (r = 0.70, P < 0.01).

Conclusion: Lateral flow assay and ELISA techniques gave consistent results for IgG/IgM antibody measurements towards spike and nucleocapsid proteins, suggesting that both methods can be used to detect COVID-19 where access to molecular test kits is difficult.

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侧流和ELISA技术检测2019冠状病毒病患者IgG和IgM抗体的评价。

背景:抗体检测可作为COVID-19分子检测的补充。目的:评价侧流法和酶联免疫吸附法(ELISA)同时检测严重急性呼吸综合征冠状病毒-2 (SARS-CoV-2)抗体的可行性。方法:本研究在日本 kocaaeli大学进行。我们使用横向流动试验和ELISA检测COVID-19病例的血清样本，并通过聚合酶链反应试验(研究组)和大流行前储存的血清样本(对照组)进行确认。我们使用Deming回归来评估抗体测量。结果:研究组纳入100例COVID-19病例，对照组纳入156例大流行前样本。横向流动试验在35和37个研究组样本中检测免疫球蛋白M (IgM)和G (IgG)抗体。ELISA检测到IgM核衣壳(N)抗体18份，IgG (N)抗体31份，IgG spike 1 (S1)抗体29份。这些技术都没有在对照样品中检测到抗体。侧流IgG (N+受体结合域+ S1)与ELISA IgG (S) (r = 0.93, P < 0.01)、ELISA IgG (N) (r = 0.81, P < 0.01)有较强的相关性。ELISA IgG S和IgG N与侧流法和ELISA IgM (N)的相关性较弱(r = 0.79, P < 0.01)。结论:横向流动法和ELISA技术对刺突蛋白和核衣壳蛋白的IgG/IgM抗体检测结果一致，表明这两种方法均可用于难以获得分子检测试剂盒的COVID-19检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Eastern Mediterranean Health Journal HEALTH CARE SCIENCES & SERVICESPUBLIC, ENV-PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

CiteScore

3.30

自引率

4.80%

发文量

112

期刊介绍： The Eastern Mediterranean Health Journal, established in 1995, is the flagship health periodical of the World Health Organization Regional Office for the Eastern Mediterranean. The mission of the Journal is to contribute to improving health in the Eastern Mediterranean Region by publishing and publicising quality health research and information with emphasis on public health and the strategic health priorities of the Region. It aims to: further public health knowledge, policy, practice and education; support health policy-makers, researchers and practitioners; and enable health professionals to remain informed of developments in public health. The EMHJ: -publishes original peer-reviewed research and reviews in all areas of public health of relevance to the Eastern Mediterranean Region -encourages, in particular, research related to the regional health priorities, namely: health systems strengthening; emergency preparedness and response; communicable diseases; noncommunicable diseases and mental health; reproductive, maternal, child health and nutrition -provides up-to-date information on public health developments with special reference to the Region. The Journal addresses all members of the health profession, health educational institutes, as well as governmental and nongovernmental organizations in the area of public health within and outside the Region.