Bioinformatics Analysis of the Inflammation-Associated lncRNA-mRNA Coexpression Network in Type 2 Diabetes.

IF 2.1 4区医学 Q3 PERIPHERAL VASCULAR DISEASE

Journal of the Renin-Angiotensin-Aldosterone System Pub Date : 2023-02-22 eCollection Date: 2023-01-01 DOI:10.1155/2023/6072438

Linjuan Huang, Shengxi Xiong, Hanshuang Liu, Min Li, Ranran Zhang, Yan Liu, Xiaolei Hu

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引用次数: 0

Abstract

Introduction: Diabetes is a chronic inflammatory state, and a key role of lncRNAs in diabetes complications is a new area of research.

Methods: In this study, key lncRNAs related to diabetes inflammation were identified by RNA-chip mining and lncRNA-mRNA coexpression network construction and finally verified by RT-qPCR.

Results: We ultimately obtained 12 genes, including A1BG-AS1, AC084125.4, RAMP2-AS1, FTX, DBH-AS1, LOXL1-AS1, LINC00893, LINC00894, PVT1, RUSC1-AS1, HCG25, and ATP1B3-AS1. RT-qPCR assays verified that LOXL1-AS1, A1BG-AS1, FTX, PVT1, and HCG25 were upregulated in the HG+LPS-induced THP-1 cells, and LINC00893, LINC00894, RUSC1-AS1, DBH-AS1, and RAMP2-AS1 were downregulated in the HG+LPS-induced THP-1 cells.

Conclusions: lncRNAs and mRNAs are extensively linked and form a coexpression network, and lncRNAs may influence the development of type 2 diabetes by regulating the corresponding mRNAs. The ten key genes obtained may become biomarkers of inflammation in type 2 diabetes in the future.

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2型糖尿病患者炎症相关lncRNA-mRNA共表达网络的生物信息学分析》（Bioinformatics Analysis of Inflammation-Associated lncRNA-mRNA Coexpression Network in Type 2 Diabetes）。

引言糖尿病是一种慢性炎症状态，lncRNA在糖尿病并发症中的关键作用是一个新的研究领域：方法：本研究通过RNA芯片挖掘和lncRNA-mRNA共表达网络构建，最终通过RT-qPCR验证了与糖尿病炎症相关的关键lncRNA：结果：我们最终获得了12个基因，包括A1BG-AS1、AC084125.4、RAMP2-AS1、FTX、DBH-AS1、LOXL1-AS1、LINC00893、LINC00894、PVT1、RUSC1-AS1、HCG25和ATP1B3-AS1。RT-qPCR 检测证实，LOXL1-AS1、A1BG-AS1、FTX、PVT1 和 HCG25 在 HG+LPS 诱导的 THP-1 细胞中上调，而 LINC00893、LINC00894、RUSC1-AS1、DBH-AS1 和 RAMP2-AS1 在 HG+LPS 诱导的 THP-1 细胞中下调。结论：lncRNA与mRNA之间存在广泛联系并形成共表达网络，lncRNA可能通过调控相应的mRNA影响2型糖尿病的发病。所获得的十个关键基因未来可能成为2型糖尿病炎症的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Renin-Angiotensin-Aldosterone System 医学-外周血管病

CiteScore

6.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： JRAAS is a peer-reviewed, open access journal, serving as a resource for biomedical professionals, primarily with an active interest in the renin-angiotensin-aldosterone system in humans and other mammals. It publishes original research and reviews on the normal and abnormal function of this system and its pharmacology and therapeutics, mostly in a cardiovascular context but including research in all areas where this system is present, including the brain, lungs and gastro-intestinal tract.