The Post-insertion Method for the Preparation of PEGylated Liposomes.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2023-01-01 DOI:10.1007/978-1-0716-2954-3_14

Sherif E Emam, Nehal E Elsadek, Taro Shimizu, Tatsuhiro Ishida

引用次数: 0

Abstract

PEGylation is a crucial process for decorating the surface of liposomes with polyethylene glycol (PEG) for clinical use. This process endows the liposomes extended circulation time and improved stability in vivo. The post-insertion method is one of the well-established techniques for PEGylation. This method requires only one-step incubation to accomplish the transfer of PEGylated lipids from PEGylated lipid-based micelles into the membranes of preformed liposomes.

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聚乙二醇化脂质体的插入后制备方法。

聚乙二醇修饰是临床上用聚乙二醇修饰脂质体表面的关键过程。这一过程延长了脂质体的循环时间，提高了体内稳定性。后插入法是一种成熟的聚乙二醇化技术。这种方法只需要一步的孵育，以完成从聚乙二醇化脂基胶束的聚乙二醇化脂转移到预形成的脂质体的膜。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.