LEPTIN RECEPTORS EXPRESSION IN MAMMARY TUMORS AND MAMMARY FAT PAD OF TRANSGENIC MAMMARY CANCER MOUSE MODEL.

Q3 Medicine

Experimental oncology Pub Date : 2022-12-01 DOI:10.32471/exp-oncology.2312-8852.vol-44-no-4.18941

Elif Yilmaz, Pinar Thomas, Bilge Tuna, Margot Cleary, Soner Dogan

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引用次数: 0

Abstract

Background: Leptin is an adipokine encoded by the Ob (obese) gene and predominantly produced by adipocytes. The roles of both leptin and leptin receptor (ObR) in numerous pathophysiological conditions including mammary tumor (MT) development have been reported.

Aim: To examine protein expression levels of leptin and its receptors (ObR) including the long form, ObRb, in MT tissue and mammary fat pad of a transgenic mammary cancer mouse model. Further, we investigated whether the effects of leptin on MT development are systemic or local.

Materials and methods: MMTV-TGF-α transgenic female mice were fed ad libitum from week 10 up to week 74. Protein expression levels of leptin, ObR, and ObRb were measured in the mammary tissue samples of 74-week old MMTV-TGF-α mice with and without MT (MT-positive/MT-negative) by Western blot analysis. Serum leptin levels were measured by using the mouse adipokine LINCOplex kit 96-well plate assay.

Results: Protein expression levels of ObRb were significantly lower in MT as compared to control tissue of mammary gland. In addition, protein expression levels of leptin were significantly higher in the MT tissue of MT-positive mice compared to control tissue of MT-negative mice. However, ObR protein expression levels in tissues of mice with and without MT were similar. Serum leptin levels at different ages were not significantly different between the two groups.

Conclusion: Leptin and ObRb in the mammary tissue may play a critical role in the mammary cancer development, while contribution of short ObR isoform may be less important.

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瘦素受体在乳腺肿瘤及乳腺脂肪垫中的表达。

背景:瘦素是一种由肥胖基因编码的脂肪因子，主要由脂肪细胞产生。瘦素和瘦素受体(ObR)在包括乳腺肿瘤(MT)发展在内的许多病理生理条件中的作用已被报道。目的:检测瘦素及其受体(ObR)在转基因乳腺癌小鼠MT组织和乳腺脂肪垫中的蛋白表达水平。此外，我们研究了瘦素对MT发展的影响是全身的还是局部的。材料与方法:MMTV-TGF-α转基因雌性小鼠从第10周至第74周自由喂养。Western blot检测74周龄MMTV-TGF-α小鼠乳腺组织中MT阳性和MT阴性(MT阳性/MT阴性)的瘦素、ObR、ObRb蛋白表达水平。采用小鼠脂肪因子LINCOplex试剂盒96孔板法测定血清瘦素水平。结果:与乳腺对照组织相比，MT中ObRb蛋白表达水平明显降低。此外，MT阳性小鼠MT组织中瘦素的蛋白表达水平明显高于MT阴性小鼠的对照组织。然而，在有MT和没有MT的小鼠组织中，ObR蛋白的表达水平是相似的。两组不同年龄段的血清瘦素水平差异无统计学意义。结论:乳腺组织中瘦素和ObRb可能在乳腺癌的发生发展中起关键作用，而短ObR异构体的贡献可能不那么重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental oncology Medicine-Oncology

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The Experimental Oncology is an English-language journal that publishes review articles, original contributions, short communications, case reports and technical advances presenting new data in the field of experimental and fundamental oncology. Manuscripts should be written in English, contain original work, which has not been published or submitted for publication elsewhere. It also implies the transfer of the Copyright from the author to “Experimental Oncology”. No part of journal publications may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the prior permission of the publisher.