Dysregulation of complement factor H in juvenile-onset systemic lupus erythematosus patients.

IF 1.1 4区 医学 Q4 Medicine
Eman Eissa, Botros Morcos, Dalia Dorgham, Naglaa Kholoussi
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Abstract

Objectives: This study aims to evaluate the expression pattern of factor H in peripheral blood and the frequency of factor H autoantibodies in plasma of juvenile-onset systemic lupus erythematosus (jSLE) patients compared to healthy controls.

Patients and methods: Between March 2019 and October 2019, a total of 30 healthy individuals (3 males, 27 females; mean age: 26±7.4 years; range, 18 to 40 years) and 65 jSLE patients (age of onset ≤16 years) (2 males, 63 females; mean age: 23.4±7 years; range, 15 to 38 years) were included. Factor H expression pattern was examined in blood of all subjects using quantitative real-time polymerase chain reaction and the frequency of factor H autoantibodies was estimated in plasma using enzyme-linked immunosorbent assay.

Results: Factor H expression was significantly downregulated in jSLE patients compared to healthy controls (p<0.01). A significant underexpression of factor H was observed in jSLE patients with nephritis compared to those without nephritis (p<0.03), while there was no association of factor H expression levels with any of the other clinical and serological features, disease activity or disease damage index of patients. Only 5% of jSLE patients were positive for factor H autoantibodies without any correlations with the clinical data or disease activity of patients.

Conclusion: Our study results suggest that factor H expression can be dysregulated in jSLE patients.

Abstract Image

补体因子H在青少年系统性红斑狼疮患者中的失调。
目的:本研究旨在评价少年型系统性红斑狼疮(jSLE)患者外周血中H因子的表达模式及血浆中H因子自身抗体的频率。患者与方法:2019年3月- 2019年10月,共30例健康个体(男3例,女27例;平均年龄:26±7.4岁;年龄18 ~ 40岁),jSLE患者65例(发病年龄≤16岁)(男性2例,女性63例;平均年龄23.4±7岁;范围,15至38岁)。使用实时定量聚合酶链反应检测所有受试者血液中因子H的表达模式,并使用酶联免疫吸附法估计血浆中因子H自身抗体的频率。结果:与健康对照组相比,jSLE患者中因子H表达明显下调(结论:我们的研究结果表明,jSLE患者中因子H表达可能失调。
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来源期刊
Archives of rheumatology
Archives of rheumatology Medicine-Rheumatology
CiteScore
2.00
自引率
9.10%
发文量
15
期刊介绍: The Archives of Rheumatology is an official journal of the Turkish League Against Rheumatism (TLAR) and is published quarterly in March, June, September, and December. It publishes original work on all aspects of rheumatology and disorders of the musculoskeletal system. The priority of the Archives of Rheumatology is to publish high-quality original research articles, especially in inflammatory rheumatic disorders. In addition to research articles, brief reports, reviews, editorials, letters to the editor can also be published. It is an independent peer-reviewed international journal printed in English. Manuscripts are refereed by a "double-blind peer-reviewed" process for both referees and authors. Editorial Board of the Archives of Rheumatology works under the principles of The World Association of Medical Editors (WAME), the International Council of Medical Journal Editors (ICMJE), and Committee on Publication Ethics (COPE).
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