DNA Methylation Trajectories During Pregnancy.

IF 3.2 Q2 GENETICS & HEREDITY
Olena Gruzieva, Simon Kebede Merid, Su Chen, Nandini Mukherjee, Anna M Hedman, Catarina Almqvist, Ellika Andolf, Yu Jiang, Juha Kere, Annika Scheynius, Cilla Söderhäll, Vilhelmina Ullemar, Wilfried Karmaus, Erik Melén, Syed Hasan Arshad, Göran Pershagen
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引用次数: 23

Abstract

There is emerging evidence on DNA methylation (DNAm) variability over time; however, little is known about dynamics of DNAm patterns during pregnancy. We performed an epigenome-wide longitudinal DNAm study of a well-characterized sample of young women from the Swedish Born into Life study, with repeated blood sampling before, during and after pregnancy (n = 21), using the Illumina Infinium MethylationEPIC array. We conducted a replication in the Isle of Wight third-generation birth cohort (n = 27), using the Infinium HumanMethylation450k BeadChip. We identified 196 CpG sites displaying intra-individual longitudinal change in DNAm with a false discovery rate (FDR) P < .05. Most of these (91%) showed a decrease in average methylation levels over the studied period. We observed several genes represented by ⩾3 differentially methylated CpGs: HOXB3, AVP, LOC100996291, and MicroRNA 10a. Of 36 CpGs available in the replication cohort, 17 were replicated, all but 2 with the same direction of association (replication P < .05). Biological pathway analysis demonstrated that FDR-significant CpGs belong to genes overrepresented in metabolism-related pathways, such as adipose tissue development, regulation of insulin receptor signaling, and mammary gland fat development. These results contribute to a better understanding of the biological mechanisms underlying important physiological alterations and adaptations for pregnancy and lactation.

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怀孕期间DNA甲基化轨迹。
关于DNA甲基化(DNAm)随时间变化的证据越来越多;然而,人们对孕期dna模式的动态变化知之甚少。我们使用Illumina Infinium MethylationEPIC阵列,对瑞典出生生命研究中具有良好特征的年轻女性样本进行了一项全表观基因组纵向dna研究,并在怀孕前、怀孕期间和怀孕后(n = 21)进行了重复的血液采样。我们使用Infinium HumanMethylation450k BeadChip在怀特岛第三代出生队列(n = 27)中进行了复制。我们确定了196个CpG位点显示个体内dna纵向变化,错误发现率(FDR)为P HOXB3、AVP、LOC100996291和MicroRNA 10a。在复制队列中可用的36个CpGs中,17个被复制,除2个外,其余均具有相同的关联方向(复制P
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来源期刊
Epigenetics Insights
Epigenetics Insights GENETICS & HEREDITY-
CiteScore
5.10
自引率
0.00%
发文量
10
审稿时长
8 weeks
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