Magnetic transferrin nanoparticles (MTNs) assay as a novel isolation approach for exosomal biomarkers in neurological diseases.

IF 11.3 1区 医学 Q1 Medicine
Yoon Ok Jang, Hee-Sung Ahn, Thuy Nguyen Thi Dao, JeongYeon Hong, Wangyong Shin, Young-Min Lim, Sun Ju Chung, Jae-Hong Lee, Huifang Liu, Bonhan Koo, Myoung Gyu Kim, Kyunggon Kim, Eun-Jae Lee, Yong Shin
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引用次数: 5

Abstract

Background: Brain-derived exosomes released into the blood are considered a liquid biopsy to investigate the pathophysiological state, reflecting the aberrant heterogeneous pathways of pathological progression of the brain in neurological diseases. Brain-derived blood exosomes provide promising prospects for the diagnosis of neurological diseases, with exciting possibilities for the early and sensitive diagnosis of such diseases. However, the capability of traditional exosome isolation assays to specifically isolate blood exosomes and to characterize the brain-derived blood exosomal proteins by high-throughput proteomics for clinical specimens from patients with neurological diseases cannot be assured. We report a magnetic transferrin nanoparticles (MTNs) assay, which combined transferrin and magnetic nanoparticles to isolate brain-derived blood exosomes from clinical samples.

Methods: The principle of the MTNs assay is a ligand-receptor interaction through transferrin on MTNs and transferrin receptor on exosomes, and electrostatic interaction via positively charged MTNs and negatively charged exosomes to isolate brain-derived blood exosomes. In addition, the MTNs assay is simple and rapid (< 35 min) and does not require any large instrument. We confirmed that the MTNs assay accurately and efficiently isolated exosomes from serum samples of humans with neurodegenerative diseases, such as dementia, Parkinson's disease (PD), and multiple sclerosis (MS). Moreover, we isolated exosomes from serum samples of 30 patients with three distinct neurodegenerative diseases and performed unbiased proteomic analysis to explore the pilot value of brain-derived blood protein profiles as biomarkers.

Results: Using comparative statistical analysis, we found 21 candidate protein biomarkers that were significantly different among three groups of neurodegenerative diseases.

Conclusion: The MTNs assay is a convenient approach for the specific and affordable isolation of extracellular vesicles from body fluids for minimally-invasive diagnosis of neurological diseases.

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磁性转铁蛋白纳米颗粒(MTNs)检测是一种分离神经疾病外泌体生物标志物的新方法。
背景:释放到血液中的脑源性外泌体被认为是研究病理生理状态的液体活检,反映了神经系统疾病中大脑病理进展的异常异质途径。脑源性血外泌体为神经系统疾病的诊断提供了良好的前景,为此类疾病的早期和敏感诊断提供了令人兴奋的可能性。然而,不能保证传统的外泌体分离方法能够特异性地分离血液外泌体,并通过高通量蛋白质组学对神经系统疾病患者临床标本的脑源性血外泌体蛋白进行表征。我们报道了磁性转铁蛋白纳米颗粒(MTNs)测定,将转铁蛋白和磁性纳米颗粒结合,从临床样品中分离脑源性血液外泌体。方法:mtn检测的原理是通过mtn上的转铁蛋白和外泌体上的转铁蛋白受体进行配体-受体相互作用,通过带正电的mtn和带负电的外泌体进行静电相互作用,分离脑源性血外泌体。结果:通过比较统计分析,我们发现21个候选蛋白质生物标志物在三组神经退行性疾病中存在显著差异。结论:MTNs检测是一种方便的方法,可以从体液中特异性分离细胞外囊泡,用于神经系统疾病的微创诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
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