Troponin T1 Promotes the Proliferation of Ovarian Cancer by Regulating Cell Cycle and Apoptosis.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Iranian Journal of Biotechnology Pub Date : 2023-01-01 DOI:10.30498/ijb.2022.344921.3405

Yuling Li, Jinfeng Qu, Yaping Sun, Chunxiao Chang

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引用次数: 0

Abstract

Background: Troponin T1 (TNNT1) is implicated in human carcinogenesis. However, the role of TNNT1 in ovarian cancer (OC) remains unclear.

Objectives: To investigate the effect of TNNT1 on the progression of ovarian cancer.

Materials and methods: The level of TNNT1 was evaluated in OC patients based on The Cancer Genome Atlas (TCGA). Knockdown or overexpression of TNNT1 using siRNA targeting TNNT1 or plasmid carrying TNNT1 was performed in the ovarian cancer SKOV3 cell, respectively. RT-qPCR was performed to detect mRNA expression. Western blotting was used to examine protein expression. Cell Counting Kit-8, colony formation, cell cycle, and transwell assays were performed to analyze the role of TNNT1 on the proliferation and migration of ovarian cancer. Besides, xenograft model was carried out to evaluate the in vivo effect of TNNT1 on OC progression.

Results: Based on available bioinformatics data in TCGA, we found that TNNT1 was overexpressed in ovarian cancer samples comparing to normal samples. Knocking down TNNT1 repressed the migration as well as the proliferation of SKOV3 cells, while overexpression of TNNT1 exhibited opposite effect. In addition, down-regulation of TNNT1 hampered the xenografted tumor growth of SKOV3 cells. Up-regulation of TNNT1 in SKOV3 cells induced the expression of Cyclin E1 and Cyclin D1, promoted cell cycle progression, and also suppressed the activity of Cas-3/Cas-7.

Conclusions: In conclusion, TNNT1 overexpression promotes SKOV3 cell growth and tumorigenesis by inhibiting cell apoptosis and accelerating cell-cycle progression. TNNT1 might be a potent biomarker for the treatment of ovarian cancer.

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肌钙蛋白T1通过调节细胞周期和凋亡促进卵巢癌增殖。

背景:肌钙蛋白T1 (TNNT1)与人类癌变有关。然而，TNNT1在卵巢癌(OC)中的作用尚不清楚。目的:探讨TNNT1在卵巢癌进展中的作用。材料与方法:基于癌症基因组图谱(TCGA)评估肿瘤患者TNNT1水平。在卵巢癌SKOV3细胞中分别使用靶向TNNT1的siRNA或携带TNNT1的质粒敲低或过表达TNNT1。RT-qPCR检测mRNA表达。Western blotting检测蛋白表达。通过细胞计数试剂盒-8、集落形成、细胞周期和transwell实验分析TNNT1在卵巢癌增殖和迁移中的作用。此外，采用异种移植模型来评估TNNT1对OC进展的体内影响。结果:基于TCGA现有的生物信息学数据，我们发现与正常样本相比，TNNT1在卵巢癌样本中过表达。敲低TNNT1抑制SKOV3细胞的迁移和增殖，而过表达TNNT1则表现出相反的作用。此外，TNNT1的下调抑制了SKOV3细胞的异种移植肿瘤生长。上调SKOV3细胞TNNT1可诱导Cyclin E1和Cyclin D1的表达，促进细胞周期进程，抑制cas3 / cas7活性。结论:综上所述，TNNT1过表达通过抑制细胞凋亡和加速细胞周期进程促进SKOV3细胞生长和肿瘤发生。TNNT1可能是卵巢癌治疗的有效生物标志物。

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来源期刊

Iranian Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

7.70%

发文量

期刊介绍： Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.