Effect of Conjugated Linoleic Acid (CLA) Supplementation on Expression of B-Cell Lymphoma-2 (Bcl-2) in the Bladder Epithelium of Wistar (Rattus norvegicus) Rats Exposed to Cigarette Smoke.

Q2 Medicine
Besut Daryanto, Edi Wibowo, Kurnia Penta Seputra, Hendy Setyo Yudhanto
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引用次数: 0

Abstract

Background: Bladder carcinoma is the 10th most common cancer in the world with an incidence about 3% of all cancers. The risk factor for smoking is found in 81% of all cases of bladder carcinoma. One of the protein groups associated with bladder urothelial carcinoma is B-Cell Lymphoma-2 (Bcl-2). Nicotine-derived nitrosamine ketone (NNK) contained in cigarette smoke would increase the proliferation of cancer cells through increased the expression of Bcl-2. The expression of Bcl-2 could be suppressed in the presence of Conjugated Linoleic Acid (CLA), a polyunsaturated fatty acid that has role in reducing the risk of cancer development which is reported in several studies, and then stimulate cell apoptosis.

Objective: To determine the effect of CLA supplementation on Bcl-2 expression in the bladder of rats which is exposed to cigarette smoke.

Methods: The study is an experimental study with true experimental posttest only control group design on Wistar rats. Sample was divided into 2 case groups: 0.5% of diet (125 mg) CLA supplementation in group A, 1% of diet (250 mg) CLA in group B; and 2 control groups: group without CLA supplementation (group C) as positive control and without cigarette smoke exposure (group D) as negative control. The study takes 60 days of exposure and then Bcl-2 expression on bladder epithelial was evaluated by immunohistochemistry staining.

Results: The results descriptively showed that rats in group C has an average Bcl-2 expression of 25.8±7.33%, while rats in group D has an average Bcl-2 expression 14.1±7.73% which means cigarette smoke exposure has been shown to increase the expression of Bcl-2 by 45.35% (p=0.019) in the bladder mucosa of experimental animals. Group B obtained an average Bcl-2 expression was 14.2±9.6% and has a significant difference when compared to group C, it shows that the addition of 1% CLA would reduce the expression of Bcl-2 by 44.96% (p=0.032). However, for group A, group with 0.5% diet of CLA supplementation did not showed decrease of Bcl-2 expression when compared to the group C (p=0.37).

Conclusion: Conjugated Linoleic Acid (CLA) supplementation 1% of diet can reduce Bcl-2 expression in bladder epithelium of wistar rats (Rattus norvegicus) exposed to cigarette smoke.

Abstract Image

Abstract Image

补充共轭亚油酸(CLA)对吸烟褐家鼠膀胱上皮b细胞淋巴瘤-2 (Bcl-2)表达的影响
背景:膀胱癌是世界上第十大最常见的癌症,发病率约占所有癌症的3%。在所有膀胱癌病例中,吸烟的危险因素占81%。与膀胱尿路上皮癌相关的蛋白群之一是b细胞淋巴瘤-2 (Bcl-2)。香烟烟雾中含有的尼古丁衍生的亚硝胺酮(NNK)会通过增加Bcl-2的表达来促进癌细胞的增殖。共轭亚油酸(Conjugated Linoleic Acid, CLA)是一种多不饱和脂肪酸,具有降低癌症发生风险的作用,有多项研究报道,共轭亚油酸可以抑制Bcl-2的表达,从而刺激细胞凋亡。目的:探讨补充CLA对吸烟大鼠膀胱Bcl-2表达的影响。方法:采用Wistar大鼠真实实验后测纯对照组设计进行实验研究。将样品分为2个病例组:A组在饲粮中添加0.5% (125 mg) CLA, B组在饲粮中添加1% (250 mg) CLA;2个对照组:未添加CLA组(C组)为阳性对照组,未接触香烟烟雾组(D组)为阴性对照组。免疫组化染色法检测膀胱上皮细胞Bcl-2的表达。结果:描述性结果显示,C组大鼠Bcl-2的平均表达量为25.8±7.33%,D组大鼠Bcl-2的平均表达量为14.1±7.73%,表明吸烟暴露使实验动物膀胱黏膜Bcl-2的表达增加了45.35% (p=0.019)。B组Bcl-2的平均表达量为14.2±9.6%,与C组相比差异有统计学意义,表明添加1%的CLA可使Bcl-2的表达量降低44.96% (p=0.032)。而在A组中,饲粮添加0.5% CLA组Bcl-2的表达与C组相比没有降低(p=0.37)。结论:饲粮中添加1%共轭亚油酸(CLA)可降低暴露于香烟烟雾的褐家鼠膀胱上皮中Bcl-2的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Medicinski arhiv
Medicinski arhiv Medicine-Medicine (all)
CiteScore
2.10
自引率
0.00%
发文量
54
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