Separating and Purifying Mycosporine-like Amino Acids from Cyanobacteria for Application in Commercial Sunscreen Formulations.

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioTech Pub Date : 2023-02-03 DOI:10.3390/biotech12010016
Valeria Candelo, Carole Anne Llewellyn
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引用次数: 1

Abstract

Using algal-derived mycosporine-like amino acids (MAAs) in sunscreen formulations is constrained by low cellular concentrations of MAAs and by the high costs associated with harvesting algal cells and extracting the MAAs. Here, we report an industrial scalable method using a membrane filtration approach to purify and concentrate aqueous extracts of MAAs. The method includes an additional biorefinery step enabling purification of phycocyanin, an established valuable natural product. Cultivated cells of the cyanobacterium Chlorogloeopsis fritschii (PCC 6912) were concentrated and homogenised to produce a feed for sequential processing through three membranes of decreasing pore size to obtain a retentate and permeate for each step. Microfiltration (0.2 µm) was used to remove cell debris. Ultrafiltration (10,000 Da) was used to remove large molecules and recover phycocyanin. Finally, nanofiltration (300-400 Da) was used to remove water and other small molecules. Permeate and retentate were analysed using UV-visible spectrophotometry and HPLC. The initial homogenised feed had a shinorine concentration of 5.6 ± 07 mg L-1. The final nanofiltered retentate resulted in a 3.3 times-purified concentrate (shinorine concentration of 18.71 ± 0.29 mg L-1). Significant process losses (35%) highlight scope for improvement. Results confirm the potential of membrane filtration to purify and concentrate aqueous solutions of MAAs with simultaneous separation of phycocyanin highlighting a biorefinery approach.

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从蓝藻中分离和纯化类真菌菌素氨基酸用于商业防晒配方。
在防晒霜配方中使用藻类衍生的真菌孢素样氨基酸(MAAs)受到MAAs细胞浓度低以及收获藻类细胞和提取MAAs相关的高成本的限制。在这里,我们报告了一种工业可扩展的方法,使用膜过滤方法纯化和浓缩MAAs的水提取物。该方法包括一个额外的生物精炼步骤,可以纯化藻蓝蛋白,这是一种已确定的有价值的天然产物。培养的蓝细菌fritschii绿藻(PCC 6912)细胞被浓缩和均质,生产出一种饲料,通过三层孔径逐渐减小的膜进行顺序处理,以获得每个步骤的保留和渗透。微滤(0.2µm)去除细胞碎片。采用超滤(10000 Da)去除大分子,回收藻蓝蛋白。最后,采用纳滤(300-400 Da)去除水和其他小分子。采用紫外可见分光光度法和高效液相色谱法分析渗透物和保留物。初始匀浆饲料中辛氨酸的浓度为5.6±07 mg L-1。最终的纳滤保留物得到3.3倍纯化的浓缩物(shinorine浓度为18.71±0.29 mg L-1)。重大工艺损失(35%)突出了需要改进的范围。结果证实了膜过滤技术在纯化和浓缩MAAs水溶液的同时分离藻蓝蛋白的潜力,突出了生物精炼方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BioTech
BioTech Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
3.70
自引率
0.00%
发文量
51
审稿时长
11 weeks
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