Trimethylamine increases intestinal fatty acid absorption: in vitro studies in a Caco-2 cell culture system.

IF 2.4 Q3 NUTRITION & DIETETICS
Journal of Nutritional Science Pub Date : 2023-11-03 eCollection Date: 2023-01-01 DOI:10.1017/jns.2023.91
Catarina Rodrigues, Shámila Ismael, Inês Castela, Inês Barreiros-Mota, Maria João Almeida, Gilberto Maia Santos, Conceição Calhau, Júlio César Rocha, Ana Faria, João R Araújo
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引用次数: 0

Abstract

Although elevated blood levels of trimethylamine N-oxide (TMAO) have been associated with atherosclerosis development in humans, the role of its gut microbiota-derived precursor, TMA, in this process has not been yet deciphered. Taking this into account, and the fact that increased intestinal fatty acid absorption contributes to atherosclerosis onset and progression, this study aimed to evaluate the effect of TMA on fatty acid absorption in a cell line that mimics human enterocytes. Caco-2 cells were treated with TMA 250 μM for 24 h. Fatty acid absorption was assessed by measuring the apical-to-basolateral transport and the intracellular levels of BODIPY-C12, a fluorescently labelled fatty acid analogue. Gene expression of the main intestinal fatty acid transporters was evaluated by real-time quantitative reverse transcription PCR. Compared to control conditions, TMA increased, in a time-dependent manner and by 20-50 %, the apical-to-basolateral transport and intracellular levels of BODIPY-C12 fatty acid in Caco-2 cells. Fatty acid transport protein 4 (FATP4) and fatty acid translocase (FAT)/CD36 gene expression were not stimulated by TMA, suggesting that TMA-induced increase in fatty acid transport may be mediated by an increase in FAT/CD36 and/or FATP4 activity and/or fatty acid passive transport. This study demonstrated that TMA increases the intestinal absorption of fatty acids. Future studies are necessary to confirm if this may constitute a novel mechanism that partially explains the existing positive association between the consumption of a diet rich in TMA sources (e.g. red meat) and the increased risk of atherosclerotic diseases.

三甲胺增加肠道脂肪酸吸收:Caco-2细胞培养系统的体外研究。
虽然血液中三甲胺n -氧化物(TMAO)水平升高与人类动脉粥样硬化的发展有关,但其肠道微生物来源的前体TMA在这一过程中的作用尚未被破译。考虑到这一点,以及肠道脂肪酸吸收增加有助于动脉粥样硬化发生和进展的事实,本研究旨在评估TMA对模拟人肠细胞细胞系脂肪酸吸收的影响。用250 μM TMA处理Caco-2细胞24小时。通过测量脂肪酸的根尖向基底侧转运和BODIPY-C12(一种荧光标记的脂肪酸类似物)的细胞内水平来评估脂肪酸吸收。采用实时定量反转录PCR技术检测肠道主要脂肪酸转运蛋白的基因表达。与对照条件相比,TMA以一种时间依赖性的方式增加了Caco-2细胞中BODIPY-C12脂肪酸的顶端到基底侧运输和细胞内水平,增加了20- 50%。脂肪酸转运蛋白4 (FATP4)和脂肪酸转位酶(FAT)/CD36基因表达不受TMA刺激,提示TMA诱导的脂肪酸转运增加可能是由FAT/CD36和/或FATP4活性增加和/或脂肪酸被动转运介导的。这项研究表明,TMA增加了肠道对脂肪酸的吸收。未来的研究需要证实这是否可能构成一种新机制,部分解释富含TMA来源的饮食(如红肉)与动脉粥样硬化疾病风险增加之间存在的正相关关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Nutritional Science
Journal of Nutritional Science NUTRITION & DIETETICS-
CiteScore
3.00
自引率
0.00%
发文量
91
审稿时长
7 weeks
期刊介绍: Journal of Nutritional Science is an international, peer-reviewed, online only, open access journal that welcomes high-quality research articles in all aspects of nutrition. The underlying aim of all work should be, as far as possible, to develop nutritional concepts. JNS encompasses the full spectrum of nutritional science including public health nutrition, epidemiology, dietary surveys, nutritional requirements, metabolic studies, body composition, energetics, appetite, obesity, ageing, endocrinology, immunology, neuroscience, microbiology, genetics, molecular and cellular biology and nutrigenomics. JNS welcomes Primary Research Papers, Brief Reports, Review Articles, Systematic Reviews, Workshop Reports, Letters to the Editor and Obituaries.
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