Ionome profiling and arsenic speciation provide evidence of arsenite detoxification in rice by phosphate and arsenite-oxidizing bacteria

Abstract

Arsenite (As(III)) as the most toxic and mobile form is the dominant arsenic (As) species in flooded paddy fields, resulting in higher accumulation of As in paddy rice than other terrestrial crops. Mitigation of As toxicity to rice plant is an important way to safeguard food production and safety. In the current study, As(III)-oxidizing bacteria Pseudomonas sp. strain SMS11 was inoculated with rice plants to accelerate conversion of As(III) into lower toxic arsenate (As(V)). Meanwhile, additional phosphate was supplemented to restrict As(V) uptake by the rice plants. Growth of rice plant was significantly inhibited under As(III) stress. The inhibition was alleviated by the introduction of additional P and SMS11. Arsenic speciation showed that additional P restricted As accumulation in the rice roots via competing common uptake pathways, while inoculation with SMS11 limited As translocation from root to shoot. Ionomic profiling revealed specific characteristics of the rice tissue samples from different treatment groups. Compared to the roots, ionomes of the rice shoots were more sensitive to environmental perturbations. Both extraneous P and As(III)-oxidizing bacteria SMS11 could alleviate As(III) stress to the rice plants through promoting growth and regulating ionome homeostasis.