Detection of carrier Booroola (Fec^B) allele in BMPR1B gene of MEGA (Merino × Garut) sheep and its association with growth traits.

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal, genetic engineering & biotechnology Pub Date : 2023-02-15 DOI:10.1186/s43141-023-00475-z

Endang Tri Margawati, Widya Pintaka Bayu Putra, Muhammad Rizki, Edi Soetrisno, Herman Willem Raadsma

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Abstract

Background: Bone morphogenetic protein receptor 1B (BMPR1B) gene is one of candidate genes for reproductive and growth traits in sheep. The present study was aimed to detect the Booroola (Fec^B) allele in BMPR1B gene and its association with growth traits in MEGA (Merino × Garut) sheep. A total of 82DNA samples collected from individual lamb (mixed-sex) blood were genotyped for allelic polymorphism using a PCR-RFLP method.

Results: The PCR analysis in BMPR1B gene resulted the amplicons with size of140 bp. The RFLP analysis with AvaII restriction enzymeresultedtwo allelic types of wildtype (A/Fec⁺) and mutant or Booroola (G/Fec^B) with frequency of 0.89 and 0.11, respectively. However, the genetic diversity in BMPR1B/AvaII gene of animal studies was categorized tolow category (PIC = 0.18)and under in a genetic equilibrium (χ² = 1.25).

Conclusions: Itshowed us that carrying Fec^B allele in the heterozygous sheep were not associated with growth traits in MEGA sheep.

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美利奴×加鲁特绵羊BMPR1B基因载体布氏菌(FecB)等位基因的检测及其与生长性状的关系

背景:骨形态发生蛋白受体1B (Bone morphogenetic protein receptor 1B, BMPR1B)基因是绵羊生殖和生长性状的候选基因之一。本研究旨在检测美利奴×加鲁特绵羊BMPR1B基因中的Booroola (FecB)等位基因及其与生长性状的关系。采用聚合酶链反应- rflp方法，对采集的82份羔羊(混合性)血液样本进行等位基因多态性分型。结果:对BMPR1B基因进行PCR分析，扩增产物大小约为140 bp。用AvaII限制性内切酶进行RFLP分析，得到野生型(A/Fec+)和突变型(G/FecB)两种等位基因型，频率分别为0.89和0.11。然而，动物实验中BMPR1B/AvaII基因的遗传多样性分为以下类别(PIC = 0.18)和低于遗传平衡(χ2 = 1.25)。结论:在杂合羊中携带FecB等位基因与MEGA羊的生长性状无关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal, genetic engineering & biotechnology

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