Analysis of the Immune Response and Identification of Antibody Epitopes Against the Sigma C Protein of Avian Orthoreovirus Following Immunization with Live or Inactivated Vaccines.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
W H Dawe, D R Kapczynski, E G Linnemann, V R Gauthiersloan, H S Sellers
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引用次数: 0

Abstract

Avian orthoreoviruses are causative agents of tenosynovitis and viral arthritis in both chickens and turkeys. Current commercial reovirus vaccines do not protect against disease caused by emerging variants. Custom-made inactivated reovirus vaccines are commonly utilized to help protect commercial poultry against disease. Antibody epitopes located on the viral attachment protein, σC, involved in virus neutralization, have not been clearly identified. In this study, the S1133 vaccine strain (Genetic Cluster 1 [GC1], a GC1 field isolate (117816), and a GC5 field isolate (94826) were determined to be genetically and serologically unrelated. In addition, chickens were vaccinated with either a commercial S1133 vaccine, 117816 GC1, or 94826 GC5, and sera were used in peptide microarrays to identify linear B-cell epitopes within the σC protein. Specific-pathogen-free (SPF) chickens were vaccinated twice with either: 1) live and live, 2) inactivated and inactivated, or 3) a combination of live and inactivated vaccines. Epitope mapping was performed on individual serum samples from birds in each group using S1133, 117816, and 94826 σC sequences translated into an overlapping peptides and spotted onto microarray chips. Vaccination with a combination of live and inactivated viruses resulted in a greater number of B-cell binding sites on the outer-capsid domains of σC for 117816 and 94826, but not for S1133. In contrast, the S1133-vaccinated birds demonstrated fewer epitopes, and those epitopes were located in the stalk region of the protein. However, within each of the vaccinated groups, the highest virus-neutralization titers were observed in the live/inactivated groups. This study demonstrates differences in antibody binding sites within σC between genetically and antigenically distinct reoviruses and provides initial antigenic characterization of avian orthoreoviruses and insight into the inability of vaccine-induced antibodies to provide adequate protection against variant reovirus-induced disease.

禽正呼肠孤病毒活疫苗和灭活疫苗免疫后免疫应答分析及Sigma C蛋白抗体表位鉴定
禽正肠病毒是鸡和火鸡腱鞘炎和病毒性关节炎的病原体。目前商业化的呼肠孤病毒疫苗不能预防由新出现的变异引起的疾病。定制的呼肠孤病毒灭活疫苗通常用于帮助保护商品家禽免受疾病侵害。抗体表位位于参与病毒中和作用的病毒附着蛋白σC上,尚未被明确鉴定。本研究确定S1133疫苗株(遗传簇1 [GC1])、GC1野分离株(117816)和GC5野分离株(94826)在遗传和血清学上不相关。另外,用S1133疫苗、117816 GC1疫苗和94826 GC5疫苗接种鸡,用多肽芯片检测血清中σC蛋白的线性b细胞表位。无特定病原体(SPF)鸡接种两次疫苗:1)活疫苗和活疫苗,2)灭活疫苗和灭活疫苗,或3)活疫苗和灭活疫苗的组合。将S1133、117816和94826 σC序列翻译成重叠肽,并在微阵列芯片上进行表位定位。用活病毒和灭活病毒联合接种后,117816和94826的σC外衣壳结构域上的b细胞结合位点数量增加,而S1133则没有。相比之下,接种s1133疫苗的禽类显示出较少的表位,这些表位位于蛋白质的茎区。然而,在每个接种组中,活/灭活组观察到最高的病毒中和滴度。本研究证明了呼肠孤病毒在σC范围内的抗体结合位点的差异,提供了禽原肠孤病毒的初步抗原特征,并深入了解了疫苗诱导抗体无法提供足够的保护以抵抗变型呼肠孤病毒诱导的疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Avian Diseases
Avian Diseases 农林科学-兽医学
CiteScore
2.40
自引率
7.10%
发文量
80
审稿时长
3 months
期刊介绍: Avian Diseases is an international journal dedicated to publishing original basic or clinical research of the highest quality from various disciplines including microbiology, immunology, pathology and epidemiology. Papers on avian diseases relevant to etiology, pathogenesis, diagnosis, treatment, and control are accepted. Manuscripts dealing with avian species other than poultry will be considered only if the subject is relevant to poultry health.
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