Effects of d-ribose on human erythrocytes: Non-enzymatic glycation of hemoglobin, eryptosis, oxidative stress and energy metabolism

IF 2.1 4区医学 Q3 HEMATOLOGY

Blood Cells Molecules and Diseases Pub Date : 2023-03-01 DOI:10.1016/j.bcmd.2023.102725

Zehong Zhang , Yu Tai , Zhi Liu , Yunxia Pu , Liang An , Xiaojing Li , Lili Li , Yaqi Wang , Zhongbin Yang , Chao Duan , Kun Hou , Qing Zhang , Fuyu Ren , Qiang Ma , Yan Su

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引用次数: 1

Abstract

d-Ribose is not only an important component of some biomacromolecules, but also an active pentose with strong reducibility and non-enzymatic glycation ability. Previous studies reported the diverse role of d-ribose in different cells. In this study, the effects of d-ribose on non-enzymatic glycation of hemoglobin (Hb), as well as eryptosis, oxidative stress and energy metabolism of erythrocytes were observed by molecular fluorescence spectrophotometry, multi-wavelength spectrophotometry, high-pressure liquid chromatography (HPLC), mass spectrometry (MS) and flow cytometer. The results showed that d-ribose had the strongest non-enzymatic glycation ability to Hb in vitro when compared with other monosaccharides, and could enter the erythrocytes in a concentration-dependent manner, which was not inhibited by the specific glucose transporter 1 (GLUT1) inhibitor WZB117. In addition, d-ribose incubation increased the HbA1c, hemolysis, eryptosis, and ROS level of erythrocytes significantly more than that of d-glucose, however, no changes were observed in the levels of ATP, NADPH, and other intermediate energy metabolites in d-ribose treatment. Therefore, the strong non-enzymatic glycation ability of d-ribose may play an important role in erythrocyte damage.

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d-核糖对人红细胞的影响:血红蛋白的非酶糖化、红细胞凋亡、氧化应激和能量代谢

d-核糖不仅是某些生物大分子的重要组成部分，而且是一种具有较强还原性和非酶糖化能力的活性戊糖。先前的研究报道了d-核糖在不同细胞中的不同作用。本研究采用分子荧光分光光度法、多波长分光光度计、高压液相色谱法、质谱法和流式细胞仪观察了d-核糖对血红蛋白（Hb）非酶糖化以及红细胞的丹毒、氧化应激和能量代谢的影响。结果表明，与其他单糖相比，d-核糖在体外对Hb的非酶糖化能力最强，并且可以以浓度依赖的方式进入红细胞，而特异性葡萄糖转运蛋白1（GLUT1）抑制剂WZB117不抑制这种能力。此外，与d-葡萄糖相比，d-核糖孵育显著增加了红细胞的HbA1c、溶血、丹毒和ROS水平，然而，在d-核糖处理中，ATP、NADPH和其他中间能量代谢产物的水平没有变化。因此，d-核糖强大的非酶糖化能力可能在红细胞损伤中发挥重要作用。

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来源期刊

Blood Cells Molecules and Diseases 医学-血液学

CiteScore

4.90

自引率

0.00%

发文量

审稿时长

14 days

期刊介绍： Blood Cells, Molecules & Diseases emphasizes not only blood cells, but also covers the molecular basis of hematologic disease and studies of the diseases themselves. This is an invaluable resource to all those interested in the study of hematology, cell biology, immunology, and human genetics.