Human ACE-2, MCP1 and micro-RNA 146 as Novel Markers for COVID- 19 Affection and Severity.

Q3 Pharmacology, Toxicology and Pharmaceutics

Infectious disorders drug targets Pub Date : 2023-01-01 DOI:10.2174/1871526522666220829153042

Amal Ahmed Mohamed, Sherief Abd-Elsalam, Ahmed Abdelghani, Mohamed Badr Hassan, Doaa Ghaith, Omnia Ezzat, Dalia Ali El-Damasy, Norhan Nagdi Madbouli, Mohmoud Hamada, Mohamed Abdel Khalik Hepatology Gastroenterology And Infectious Diseases, Shaimaa M Al-Tabbakh, Kareman Ahmed Ebrahim Eshra, Nivin Baiomy

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引用次数: 0

Abstract

Background & aims: Coronavirus disease - 2019 (COVID-19) is a major pandemic that causes high morbidity and mortality rates.

Aim of this study: to detect the relations between many risk factors, ACE-2, MCP-1, Micro RNA 146 gene expression, and COVID-19 infection and disease severity.

Methods: This study was carried out on 165 cases of COVID-19 and 138 controls. ACE2 and MCP1 levels were measured in COVID-19 cases and control by ELISA and micro-RNA-146 expression by PCR.

Results: We found an increased blood level of ACE2 and MCP1 in COVID- 19 patients than in healthy persons and a significant down-regulation of micro-RNA 146 gene expression in cases than in controls. There was a significant correlation between increased blood level of ACE2, regulation of micro-RNA 146 gene expression and severity of lung affection, a significant correlation was found between increased blood level of MCP1 and thrombosis in COVID-19 patients. Neurological complications were significantly correlated with more viral load, more ACE2 blood level, and down regulation of micro RNA146 expression.

Conclusion: High viral load, increased blood level of ACE2, and down-regulation of micro-RNA 146 expression are associated with more severe lung injury and the presence of neurologic complications like convulsions and coma in COVID-19 Egyptian patients.

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人ACE-2、MCP1和微rna 146作为COVID- 19影响和严重程度的新标志物

背景与目的:冠状病毒病- 2019 (COVID-19)是一种导致高发病率和死亡率的重大流行病。本研究目的:检测多种危险因素、ACE-2、MCP-1、Micro RNA 146基因表达与COVID-19感染及病情严重程度的关系。方法:选取新冠肺炎病例165例，对照组138例。采用ELISA法检测COVID-19病例和对照组的ACE2和MCP1水平，采用PCR法检测micro-RNA-146的表达。结果:我们发现COVID- 19患者血液中ACE2和MCP1水平高于健康人，微rna 146基因表达明显低于对照组。新冠肺炎患者血中ACE2水平升高、微rna 146基因表达调控与肺损伤严重程度有显著相关性，MCP1水平升高与血栓形成有显著相关性。神经系统并发症与病毒载量升高、ACE2血药浓度升高、微RNA146表达下调显著相关。结论:埃及新冠肺炎患者高病毒载量、血中ACE2水平升高、微rna 146表达下调与更严重的肺损伤及惊厥、昏迷等神经系统并发症相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infectious disorders drug targets Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.10

自引率

0.00%

发文量

123

期刊介绍： Infectious Disorders - Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular targets involved in infectious disorders e.g. disease specific proteins, receptors, enzymes, genes. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics on drug targets involved in infectious disorders. As the discovery, identification, characterization and validation of novel human drug targets for anti-infective drug discovery continues to grow, this journal will be essential reading for all pharmaceutical scientists involved in drug discovery and development.