Anesthesia for Echocardiography and Magnetic Resonance Imaging in the African Clawed Frog (Xenopus laevis).

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Antonio F Corno, Noelia E Flores, Wen Li, Thomas H Gomez, Jorge D Salazar
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引用次数: 2

Abstract

This report describes an anesthesia technique that we used to study cardiovascular anatomy and physiology with echocardiography and cardiac magnetic resonance (CMR) in 46 African clawed frogs (Xenopus laevis) (n = 24 for electrocardiography and n = 22 for CMR). For administration of anesthesia, 3 holding tanks, one each for transportation, sedation, and recovery, were filled with filtered water, with 0.05% buffered tricaine methasulfonate solution (MS-222) added into the sedation tank. Fifteen minutes after the frog was placed in the sedation tank, a paper towel was soaked in MS-222 solution, and the frog was placed in a supine position and rolled 3 to 4 times in the soaked paper with the head and legs exposed. Vital signs were monitored and recorded throughout the procedure. After imagining, frogs were unrolled from the paper towel, placed in the recovery tank, and later returned to their home tank. Monitoring was discontinued when the frogs resumed typical activity. No mortality or complications were observed in frogs that underwent this procedure. Mean duration ±1 SD of anesthesia induction was 12 ± 5 min in the echocardiography group and 14 ± 6 min in the CMR group. The mean duration of anesthesia maintenance was 60 ± 18 min in the echocardiography group and 118 ± 37 min in the CMR group. An additional dose of anesthesia was necessary during maintenance for 9 of 24 (37%) frogs in the echocardiography group and 6 of 22 (27%) frogs in the CMR group. At the end of the procedure, the mean oxygen saturation was 66 ± 9% in the echocardiography group and 85 ± 6% in the CMR group, and heart rate was 48 ± 13 beats/min in the echocardiography group and 42 ± 7 beats/min in the CMR group. We conclude that the anesthesia technique of immersion in MS-222 is suitable for performing echocardiography and CMR imaging in this species without complications.

Abstract Image

非洲爪蛙超声心动图和磁共振成像的麻醉。
本报告描述了一种麻醉技术,我们用超声心动图和心脏磁共振(CMR)研究了46只非洲爪蛙(Xenopus laevis)的心血管解剖和生理(n = 24心电图和n = 22 CMR)。麻醉时,取3个保存槽,分别用于运输、镇静和恢复,每个保存槽中装满过滤水,镇静槽中加入0.05%的缓冲甲基磺酸三卡因溶液(MS-222)。将青蛙放入镇静池15分钟后,用纸巾浸泡MS-222溶液,将青蛙置于仰卧位,在浸泡过的纸中翻滚3 ~ 4次,露出头和腿。在整个过程中监测并记录生命体征。在想象之后,青蛙从纸巾中展开,放在回收水箱中,后来又回到了它们的家庭水箱。当青蛙恢复正常活动时,监测就停止了。在接受这种手术的青蛙中没有观察到死亡或并发症。超声心动图组麻醉诱导平均持续时间±1 SD为12±5 min, CMR组为14±6 min。超声心动图组平均麻醉维持时间为60±18 min, CMR组平均麻醉维持时间为118±37 min。超声心动图组24只青蛙中有9只(37%)和CMR组22只青蛙中有6只(27%)在维持过程中需要额外剂量的麻醉。手术结束时,超声心动图组平均血氧饱和度66±9%,CMR组平均血氧饱和度85±6%,心率48±13次/分,CMR组平均42±7次/分。我们认为MS-222浸泡麻醉技术适用于该物种的超声心动图和CMR成像,无并发症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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