Gallic Acid Ameliorates Cadmium Effect on Osteogenesis by Activation of Alkaline Phosphatase and Collagen Synthesis.

IF 1.7 4区生物学 Q4 CELL BIOLOGY

Cell Journal Pub Date : 2023-09-09 DOI:10.22074/cellj.2023.1999110.1263

Mohammad Hossein Abnosi, Javad Sargolzaei, Farshid Nazari

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引用次数: 0

Abstract

Objective: We previously reported that cadmium (Cd) inhibits osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). In addition, gallic acid (GA) improves BMSC differentiation. Here, we aim to study the ability of GA to prevent osteogenic inhibition induced by Cd.

Materials and methods: In this experimental study, BMSCs were extracted and purified from Wistar rats and their viability was determined in the presence of Cd and GA. The results indicated that 1.5 μM Cd and 0.25 μM of GA were appropriate for further investigation. After 20 days in osteogenic medium, matrix production was analysed by alizarin red, calcium content, and alkaline phosphatase (ALP) activity. Osteogenic-related genes and collagen 1A1 (COL1A1) protein expressions were investigated. The preventive effect of GA on oxidative stress and metabolic change induced by Cd was estimated.

Results: GA counteracted the inhibitory effect of Cd on matrix production and significantly (P=0.0001) improved the osteogenic differentiation ability of BMSCs. Also, GA prevented the toxic effect of Cd on osteogenic-related gene expressions and nullified the reducing effect of Cd on COL1Al and ALP activity. A significant reduction (P=0.0001) in malondialdehyde and lactic acid concentration showed that GA counteracted both oxidative stress and metabolic changes caused by Cd.

Conclusion: GA prevented the toxic effect of Cd, an environmental pollutant and a factor in osteoporosis.

Abstract Image

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没食子酸通过激活碱性磷酸酶和胶原合成改善镉对成骨的影响。

目的：我们先前报道了镉（Cd）抑制骨髓间充质干细胞（BMSCs）的成骨分化。此外，没食子酸（GA）可改善BMSC的分化。材料与方法：本实验从Wistar大鼠中提取纯化BMSCs，并在Cd和GA存在下测定其活力。结果表明，1.5μM Cd和0.25μM GA适合进一步研究。在成骨培养基中培养20天后，通过茜素红、钙含量和碱性磷酸酶（ALP）活性分析基质的产生。研究了成骨相关基因和胶原1A1（COL1A1）蛋白的表达。探讨了GA对镉引起的氧化应激和代谢变化的预防作用。结果：GA拮抗了Cd对基质产生的抑制作用，显著提高了骨髓基质干细胞的成骨分化能力（P=0.0001）。GA还阻止了Cd对成骨相关基因表达的毒性作用，并抵消了Cd对COL1Al和ALP活性的还原作用。丙二醛和乳酸浓度的显著降低（P=0.0001）表明，GA可以抵消Cd引起的氧化应激和代谢变化。结论：GA可以预防环境污染物Cd的毒性作用，Cd是骨质疏松症的一个因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Journal CELL BIOLOGY-

CiteScore

3.40

自引率

5.00%

发文量

审稿时长

12 months

期刊介绍： The “Cell Journal (Yakhteh)“, formerly published as “Yakhteh Medical Journal”, is a quarterly English publication of Royan Institute. This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.