The protective effect of curcumin on thrombin-induced hyper-permeability.

IF 1.9 Q3 CHEMISTRY, MEDICINAL
Farzad Rahmani, Hossein Abdeahad, Najmeh Jaberi, Reyhane Hanaie, Atena Soleimani, Amir Avan, Majid Khazaei, Seyed Mahdi Hassanian
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引用次数: 1

Abstract

Objective: Thrombin is a proinflammatory and pro-coagulant agent which is upregulated in several human diseases. Thrombin has a critical role in promoting cell proliferation and microvascular leakage in malignant cells, resulting in cancer growth and progression. Here, we explored the potential therapeutic value of curcumin on permeability induced by thrombin in mice.

Materials and methods: To assess the activity of curcumin on thrombin-induced vascular permeability mice model, C57BL / 6 mice were randomly divided into four groups: (1) control (2) Thrombin (3) Thrombin + Curcumin and (4) Thrombin + Metformin. Thirty minutes after treatment, Evans blue was injected intravenously through the tail vein to mice. Then, animals were sacrificed and the dye was extracted from the skin tissue by incubation with formamide. Heatmap and correlation map were generated and protein-protein interaction network of the hub genes was drawn by Cytoscape software.

Results: Hub DEG expression rate showed that Heat shock protein a1 (Hspa1) family (comprised of HSPa1a, b, and HSPa5), caspase 3, and minichromosome maintenance complex component 2 were overexpressed after treatment with curcumin. Functional modules of curcumin enriched through Enrich gene biological process and revealed positive association of gene expression of apoptosis process with the therapy. Curcumin was also found to reduce leucocyte migration in murine tissues. Additionally, treatment with curcumin resulted in downregulation of heat shock proteins and proinflammatory cytokines such as monocyte chemotactic protein 1, interleukin-6 and chemokine (C-X-C motif) ligand 3.

Conclusion: Curcumin inhibited the proinflammatory cytokines and inflammatory HSPs in endothelial cells and reduced thrombin-induced barrier destabilization in vivo.

姜黄素对凝血酶诱导的高渗透性的保护作用。
目的:凝血酶是一种促炎促凝血剂,在多种人类疾病中上调。Thrombin在促进恶性细胞的细胞增殖和微血管渗漏,导致癌症生长和进展方面具有关键作用。在此,我们探讨了姜黄素对凝血酶诱导的小鼠通透性的潜在治疗价值。材料和方法:为了评估姜黄素对凝血酶诱导的血管通透性小鼠模型的活性,将C57BL/6小鼠随机分为四组:(1)对照组(2)凝血酶组(3)凝血酶+姜黄素组和(4)凝血酶+二甲双胍组。治疗30分钟后,通过尾静脉将伊文思蓝静脉注射给小鼠。然后,处死动物,并通过与甲酰胺孵育从皮肤组织中提取染料。利用Cytoscape软件生成热图和相关图,绘制枢纽基因的蛋白质-蛋白质相互作用网络。结果:Hub-DEG表达率显示,姜黄素处理后,热休克蛋白a1(Hspa1)家族(由HSPa1a、b和HSPa5组成)、胱天蛋白酶3和微染色体维持复合物组分2过表达。姜黄素的功能模块通过富集基因生物学过程富集,并揭示了凋亡过程的基因表达与治疗呈正相关。姜黄素还被发现可以减少小鼠组织中白细胞的迁移。此外,姜黄素治疗可下调热休克蛋白和促炎细胞因子,如单核细胞趋化蛋白1、白细胞介素-6和趋化因子(C-X-C基序)配体3。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Avicenna Journal of Phytomedicine
Avicenna Journal of Phytomedicine CHEMISTRY, MEDICINAL-
CiteScore
3.40
自引率
4.50%
发文量
17
审稿时长
6 weeks
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