Sodium butyrate attenuate hyperglycemia-induced inflammatory response and renal injury in diabetic mice.

IF 2.1 4区医学 Q3 PHARMACOLOGY & PHARMACY

Acta Pharmaceutica Pub Date : 2023-03-01 DOI:10.2478/acph-2023-0008

Man Yan, Yan-Yan Zhang, Yue Xi, Long-Kun Ding, Chang Sun, Li-Juan Qu, Xin Qian, Jing-Wen Xu, Wen Sun, Liang Wu

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引用次数: 1

Abstract

The activation of the monocyte-macrophage system and the damage to the renal and pancreatic tissue are common complications in patients with diabetes induced by hyper-glycemia. This study aimed to evaluate the effect and mechanism of butyrate (NaB), a metabolite of intestinal flora, on inhibiting the inflammatory response of human monocyte-macrophages (THP-1 cells) induced by high glucose and the damage of pancreatic and renal tissue in diabetic mice. The results showed that high concentration glucose significantly up-regulated the expressions of IL-1β, TNF-α, and NLRP3 in THP-1 cells and mouse spleen, and that NaB could inhibit the overexpression of those genes. The abundance of Beclin-1, LC3B and reactive oxygen species (ROS) in THP-1 cells is increased due to the high glucose concentration, and NaB can inhibit the genes responsible for upregulating the expression. In diabetic mice, vacuolar degeneration of renal tubules was observed. Then we observed that some of the epithelial cells of the renal tubules were exfoliated and some formed tubules. NaB could alleviate these pathological lesions, but NaB cannot alleviate pancreatic injury. Our results indicated that NaB could be used for the prevention and adjuvant treatment of diabetic kidney injury.

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丁酸钠减轻糖尿病小鼠高血糖诱导的炎症反应和肾损伤。

单核-巨噬细胞系统的激活和对肾脏和胰腺组织的损害是高血糖引起的糖尿病患者常见的并发症。本研究旨在探讨肠道菌群代谢物丁酸盐(NaB)对高糖诱导的人单核巨噬细胞(THP-1细胞)炎症反应及糖尿病小鼠胰腺和肾脏组织损伤的抑制作用及其机制。结果表明，高浓度葡萄糖可显著上调THP-1细胞和小鼠脾脏中IL-1β、TNF-α和NLRP3的表达，NaB可抑制这些基因的过表达。由于高葡萄糖浓度，THP-1细胞中Beclin-1、LC3B和活性氧(ROS)的丰度增加，NaB可以抑制上调表达的基因。在糖尿病小鼠中，观察到肾小管空泡变性。我们观察到肾小管上皮细胞部分脱落，部分形成小管。NaB可减轻这些病理病变，但不能减轻胰腺损伤。提示NaB可用于糖尿病肾损伤的预防和辅助治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Pharmaceutica PHARMACOLOGY & PHARMACY-

CiteScore

5.20

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： AP is an international, multidisciplinary journal devoted to pharmaceutical and allied sciences and contains articles predominantly on core biomedical and health subjects. The aim of AP is to increase the impact of pharmaceutical research in academia, industry and laboratories. With strong emphasis on quality and originality, AP publishes reports from the discovery of a drug up to clinical practice. Topics covered are: analytics, biochemistry, biopharmaceutics, biotechnology, cell biology, cell cultures, clinical pharmacy, drug design, drug delivery, drug disposition, drug stability, gene technology, medicine (including diagnostics and therapy), medicinal chemistry, metabolism, molecular modeling, pharmacology (clinical and animal), peptide and protein chemistry, pharmacognosy, pharmacoepidemiology, pharmacoeconomics, pharmacodynamics and pharmacokinetics, protein design, radiopharmaceuticals, and toxicology.