The use of occlusive dressings: influence on excisional wound healing in animal model.

IF 1.1 4区医学 Q3 SURGERY

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI:10.1590/acb371206

Mariana Raquel Soares Guillen, Eline Lima Borges, Gilmara Lopes Amorim, Puebla Cassini Vieira, Antônio Carlos Martins Guedes, Luciola Silva Barcelos

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Abstract

Purpose: To analyze the influence of occlusive dressing on the healing of excisional skin wounds in mice.

Methods: Pre-clinical, comparative, and translational study. Mice were divided into three experimental groups: wounds occluded with hydrocolloid (HD) dressings, transparent polyurethane film (TF) dressings, and without occlusion (WO), monitored at three, six and 14 days, with eight animals each. Closure rate, infiltration of neutrophils and macrophages, measurement of tumor necrosis factor-α (TNF-α) and vascular endothelial growth factor (VEGF) and, histologically, angiogenesis were evaluated.

Results: Wound closure was accelerated in the occlusive groups. There was a decrease in TNF-α levels in the HD group when compared to the WO and TF groups. Neutrophils accumulation decreased in the HD group. Increased dosages of macrophages were evidenced in the HD group, compared to the WO and TF groups. Levels of VEGF were increased in the TF and HD groups.

Conclusions: It is suggested that the occlusion of wounds modulates the inflammatory response.

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封闭敷料对动物模型切除创面愈合的影响。

目的:分析封闭敷料对小鼠皮肤切除创面愈合的影响。方法:临床前、比较和转化研究。将小鼠分为水胶体(HD)敷料、透明聚氨酯膜(TF)敷料和不闭塞(WO) 3组，分别于第3、6和14天进行监测，每组8只。观察闭合率、中性粒细胞和巨噬细胞的浸润、肿瘤坏死因子-α (TNF-α)和血管内皮生长因子(VEGF)的测定以及组织学上的血管新生情况。结果:封闭组伤口愈合速度加快。与WO和TF组相比，HD组TNF-α水平降低。HD组中性粒细胞积累减少。与WO和TF组相比，HD组巨噬细胞剂量增加。TF组和HD组VEGF水平升高。结论:伤口封闭对炎症反应有调节作用。

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来源期刊

Acta cirurgica brasileira 医学-外科

CiteScore

1.90

自引率

9.10%

发文量

审稿时长

3-8 weeks

期刊介绍： Information not localized