Hand disinfectants and their activity against clinical isolates of Bordetella pertussis.

IF 1.1 4区医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Central European journal of public health Pub Date : 2022-12-01 DOI:10.21101/cejph.a7141

Petra Uttlová, Jan Urban

{"title":"Hand disinfectants and their activity against clinical isolates of Bordetella pertussis.","authors":"Petra Uttlová, Jan Urban","doi":"10.21101/cejph.a7141","DOIUrl":null,"url":null,"abstract":"Objectives: The aim of the study was to investigate possible emergence of resistance to disinfectants in Bordetella pertussis strains isolated from patients with whooping cough in the Czech Republic in 2014 and 2015.Methods: In an EN1500-based study, clean and dry fingertips of volunteers were always contaminated with one of the two clinical isolates of B. pertussis. Clinical isolates of B. pertussis were obtained from the National Reference Laboratory for Pertussis and Diphtheria, National Institute of Public Health (NIPH), Prague, Czech Republic. Dry and contaminated fingertips were immersed in 10 ml medium and then rubbed with the fingers for 1 minute. After that, the hands were treated with isopropanol 60% v/v or tested products, and then the fingertips were rubbed again into 10 ml of pure medium for 1 minute. The suspensions obtained were immediately diluted and plated on charcoal medium.Results: Ethanol-based product A and propanol-based product B showed bactericidal activity after 30 s of contact. The confidence interval limit for product A and B was 0.12 and 0.19, respectively. Quaternary ammonium compound-based product C was found to be ineffective after 30 s of contact. The confidence interval limit for product C was 0.62.Conclusion: Products A and B were assessed as effective against clinical isolates of B. pertussis in accordance with EN 1500. Quaternary ammonium compound-based product C did not comply with the requirements of EN 1500.","PeriodicalId":9823,"journal":{"name":"Central European journal of public health","volume":"30 4","pages":"230-234"},"PeriodicalIF":1.1000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Central European journal of public health","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.21101/cejph.a7141","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: The aim of the study was to investigate possible emergence of resistance to disinfectants in Bordetella pertussis strains isolated from patients with whooping cough in the Czech Republic in 2014 and 2015.

Methods: In an EN1500-based study, clean and dry fingertips of volunteers were always contaminated with one of the two clinical isolates of B. pertussis. Clinical isolates of B. pertussis were obtained from the National Reference Laboratory for Pertussis and Diphtheria, National Institute of Public Health (NIPH), Prague, Czech Republic. Dry and contaminated fingertips were immersed in 10 ml medium and then rubbed with the fingers for 1 minute. After that, the hands were treated with isopropanol 60% v/v or tested products, and then the fingertips were rubbed again into 10 ml of pure medium for 1 minute. The suspensions obtained were immediately diluted and plated on charcoal medium.

Results: Ethanol-based product A and propanol-based product B showed bactericidal activity after 30 s of contact. The confidence interval limit for product A and B was 0.12 and 0.19, respectively. Quaternary ammonium compound-based product C was found to be ineffective after 30 s of contact. The confidence interval limit for product C was 0.62.

Conclusion: Products A and B were assessed as effective against clinical isolates of B. pertussis in accordance with EN 1500. Quaternary ammonium compound-based product C did not comply with the requirements of EN 1500.

查看原文本刊更多论文

手部消毒剂对百日咳杆菌临床分离株的活性研究。

目的:本研究的目的是调查2014年和2015年从捷克共和国百日咳患者中分离的百日咳博德泰拉菌株对消毒剂可能出现的耐药性。方法:在一项基于en1500的研究中，志愿者的清洁和干燥的指尖总是被两种临床分离株百日咳中的一种污染。百日咳临床分离株来自捷克共和国布拉格国家公共卫生研究所(NIPH)百日咳和白喉国家参考实验室。将干燥和污染的指尖浸入10ml培养基中，然后用手指摩擦1分钟。之后，用60% v/v的异丙醇或被试产品处理双手，然后将指尖再次放入10 ml纯培养基中揉搓1分钟。得到的悬浮液立即稀释，并在木炭培养基上镀。结果:乙醇基产品A和丙醇基产品B在接触30 s后显示出杀菌活性。产品A和B的置信区间极限分别为0.12和0.19。季铵盐基产品C在接触30 s后失效。产品C的置信区间限为0.62。结论:产品A和B对临床分离株百日咳有效，符合en1500标准。季铵化合物基产品C不符合en1500的要求。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Central European journal of public health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： The Journal publishes original articles on disease prevention and health protection, environmental impacts on health, the role of nutrition in health promotion, results of population health studies and critiques of specific health issues including intervention measures such as vaccination and its effectiveness. The review articles are targeted at providing up-to-date information in the sphere of public health. The Journal is geographically targeted at the European region but will accept specialised articles from foreign sources that contribute to public health issues also applicable to the European cultural milieu.