Calicophoron daubneyi (Dinnik, 1962) (Digenea) in beef and dairy cattle in the Czech Republic: prevalence and drug efficacy.

IF 1.5 4区医学 Q3 PARASITOLOGY

Folia Parasitologica Pub Date : 2023-01-02 DOI:10.14411/fp.2023.001

Anna Bastyrova Brutovska, Pavlina Vogalova, Michael Rost, Bohumil Sak, Martin Kvac

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引用次数: 1

Abstract

A total of 1,724 beef and 2,941 dairy cattle older than one year from 66 beef and 67 dairy farms in the Czech Republic were examined for the presence of rumen and liver fluke eggs in 2019-2022. Out of 227 positive animals, all were positive for paramphistome and five for fasciolid eggs. Molecular analysis of the ITS2 rDNA revealed the presence of Calicophoron daubneyi (Dinnik, 1962) and Fasciola hepatica Linnaeus, 1758. Faecal egg count (FEC) showed low infection intensity (12 EPG) in animals infected with F. hepatica and high variability in C. daubneyi infections (2-589 EPG). Efficacy of oxyclozanide, albendazole, ivermectin, and closantel against C. daubneyi infection was evaluated at eight beef cattle herds. Faecal samples were collected from all positive animals at 0 and 21days post-treatment. Based on FEC, albendazole, ivermectin and closantel reduced the number of C. daubneyi eggs shed by 0-9.9%, with no effect on the number of infected animals. The use of oxyclozanide on two beef farms showed 100% efficacy against C. daubneyi and F. hepatica. Follow-up examination 5-6 months after drug application showed reinfection of most animals with C. daubneyi, but the FEC was significantly lower. The finding of four dairy cows infected with C. daubneyi housed in a stable without pasture suggests the possibility of the infection being introduced through roughage.

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捷克共和国牛肉和奶牛中的Calicophoron daubneyi (Dinnik, 1962) (Digenea):患病率和药物功效。

2019-2022年，对捷克共和国66个牛肉农场和67个奶牛场的1724头1岁以上的牛肉和2941头1岁以上的奶牛进行了瘤胃和肝脏吸虫蛋检查。在227只阳性的动物中，所有的动物都呈副虫阳性，5只呈筋膜类卵阳性。对ITS2 rDNA的分子分析显示存在多布尼卡利虫(Calicophoron daubneyi, Dinnik, 1962)和林奈肝片形吸虫(Linnaeus, 1758)。粪卵计数(FEC)在感染肝梭菌的动物中表现为低感染强度(12 EPG)，在感染多布尼梭菌的动物中表现为高变异性(2-589 EPG)。在8个肉牛群中评价了氧氯胺、阿苯达唑、伊维菌素和closantel对C. daubneyi感染的疗效。在治疗后0天和21天采集所有阳性动物的粪便样本。以FEC为基础，阿苯达唑、伊维菌素和closantel可使多布尼伊氏梭菌产蛋数量减少0-9.9%，对感染动物数量无影响。在两个牛肉养殖场使用氧氯胺对多布内梭菌和肝原胞菌的效果为100%。用药后5 ~ 6个月随访检查，大部分动物再次感染多布尼梭菌，但FEC明显降低。在没有牧场的马厩中发现了四头感染多布尼埃氏乳杆菌的奶牛，这表明感染可能是通过粗饲料引入的。

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来源期刊

Folia Parasitologica 医学-寄生虫学

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： FOLIA PARASITOLOGICA, issued in online versions, is an international journal that covers the whole field of general, systematic, ecological and experimental parasitology. It publishes original research papers, research notes and review articles. Contributions from all branches of animal parasitology, such as morphology, taxonomy, biology, biochemistry, physiology, immunology, molecular biology and evolution of parasites, and host-parasite relationships, are eligible. Novelty and importance in the international (not local or regional) context are required. New geographical records of parasites, records of new hosts, regional parasite and/or host surveys (if they constitute the principal substance of manuscript), local/regional prevalence surveys of diseases, local/regional studies on epidemiology of well known diseases and of parasite impact on human/animal health, case reports, routine clinical studies and testing of established diagnostic or treatment procedures, will not be considered. One species description will also not be considered unless they include more general information, such as new diagnostic characters, host-parasite associations, phylogenetic implications, etc. Manuscripts found suitable on submission will be reviewed by at least two reviewers.