Comparison of Cardioplegic Solutions in Coronary Bypass Surgery Over Autophagy and Apoptosis Mechanisms.

IF 2 4区医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS

Arquivos Brasileiros de Cardiologia Pub Date : 2023-06-01 DOI:10.36660/abc.20220479

Elif Funda Sener, Zuhal Hamurcu, Serpil Taheri, Reyhan Tahtasakal, Nesrin Delibasi, Deniz Elcik, Ecmel Mehmetbeyoglu, Aydin Tuncay, Fatma Dal, Keziban Korkmaz Bayram, Isın Gunes, Omer Naci Emirogullari

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引用次数: 0

Abstract

Background: Coronary artery disease (CAD) due to myocardial ischemia causes permanent loss of heart tissue.

Objectives: We aimed to demonstrate the possible damage to the myocardium at the molecular level through the mechanisms of autophagy and apoptosis in coronary bypass surgery patients.

Methods: One group was administered a Custodiol cardioplegia solution, and the other group was administered a Blood cardioplegia solution. Two myocardial samples were collected from each patient during the operation, just before cardiac arrest and after the aortic cross-clamp was released. The expressions of autophagy and apoptosis markers were evaluated. The level of statistical significance adopted was 5%.

Results: The expression of the BECLIN gene was significant in the myocardial tissues in the BC group (p=0.0078). CASPASE 3, 8, and 9 gene expression levels were significantly lower in the CC group. Postoperative TnT levels were significantly different between the groups (p=0.0072). CASPASE 8 and CASPASE 9 gene expressions were similar before and after aortic cross-clamping (p=0.8552, p=0.8891). In the CC group, CASPASE 3, CASPASE 8, and CASPASE 9 gene expression levels were not found to be significantly different in tissue samples taken after aortic cross-clamping (p=0.7354, p=0.0758, p=0.4128, respectively).

Conclusions: With our findings, we believe that CC and BC solutions do not have a significant difference in terms of myocardial protection during bypass operations.

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冠状动脉搭桥术中自噬和细胞凋亡机制下心脏截瘫解决方案的比较。

背景:冠状动脉疾病(CAD)是由心肌缺血引起的永久性心脏组织损失。目的:探讨冠状动脉搭桥术患者自噬和细胞凋亡在分子水平上可能对心肌造成的损伤。方法:1组给予舒迪欧尔停搏液，1组给予血停搏液。在手术中，分别在心脏骤停前和主动脉交叉夹解除后，从每位患者身上采集了两份心肌样本。观察细胞自噬和凋亡标志物的表达。采用统计学显著性水平为5%。结果:BC组心肌组织BECLIN基因表达显著(p=0.0078)。CASPASE 3、8、9基因表达水平在CC组显著降低。两组术后TnT水平差异有统计学意义(p=0.0072)。CASPASE 8和CASPASE 9基因表达在主动脉交叉夹紧前后相似(p=0.8552, p=0.8891)。在CC组中，CASPASE 3、CASPASE 8、CASPASE 9基因表达水平在主动脉交叉夹持后的组织样本中无显著差异(p=0.7354、p=0.0758、p=0.4128)。结论:根据我们的研究结果，我们认为CC和BC溶液在旁路手术期间的心肌保护方面没有显着差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Arquivos Brasileiros de Cardiologia 医学-心血管系统

CiteScore

3.70

自引率

30.80%

发文量

234

审稿时长

3-8 weeks

期刊介绍： With more than 70 years of existence, Arquivos Brasileiros de Cardiologia is the main channel for the dissemination of Brazilian scientific research on cardiovascular sciences. Published in two languages and indexed in major international databases, all scientific contributions are peer-reviewed and reviewed by editorial board members selected among the most reputable researchers in Brazil and abroad. The manuscripts are reviewed according to their relevance and originality, scientific accuracy and level of importance for the advancement of science. With an average time of nine months between the initial submission and the effective publication of the manuscripts, and less than seven months until they are published on PubMed, Arquivos Brasileiros de Cardiologia can ensure the quick inclusion of the researchers’ papers in the international literature.