Additional data on Spinitectus petterae (Nematoda: Rhabditida) from Clarias gariepinus (Siluriformes: Clariidae) in the Vaal River system: conserved morphology or high intraspecific genetic variability?

IF 1.5 4区 医学 Q3 PARASITOLOGY
Lucinda Austin, Quinton Marco Dos Santos, Annemarie Avenant-Oldewage
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引用次数: 1

Abstract

Two species of Spinitectus Fourment, 1884 have been recorded from southern Africa, namely Spinitectus polli Campana-Rouget, 1961 and Spinitectus petterae Boomker, 1993, both from the Limpopo River system. Spinitectus petterae was described from North African catfish, Clarias gariepinus (Burchell), whereas S. polli infects squeakers, Synodontis spp. During parasitological surveys in the Vaal River system (Orange River catchment), Spinitectus specimens were collected from C. gariepinus. These systems are adjacent but not connected. Therefore, this study aimed to identify the specimens collected using morphological and molecular techniques. The morphological study included light and scanning electron microscopy of whole specimens and excised spicules. Specimens were genetically characterised using 18S rDNA, 28S rDNA and cox1 mtDNA. Additionally, immature specimens of S. petterae were collected near the type locality. Morphological characteristics were most similar to S. petterae from C. gariepinus, whereas genetic data were dissimilar to all available data for the genus. Additional morphological characteristics noted for S. petterae in the present study were the details of the left and right spicule structure and the porous structures on the pseudolabia. Specimens from the Vaal River system differed from those originally described as S. petterae by additional spines posterior to the third ring, lacking caudal alae and variable total body and male oesophagus length. Based on 18S rDNA, haplotypes from the type locality varied only slightly from the study material, supporting the morphological identification. However, 28S rDNA and, more conspicuously, cox1 mtDNA displayed substantial variation between specimens from these localities, which needs further investigation. Haplotypes generated in the present study were highly dissimilar to those characterised for S. petterae from Tanzania and Egypt. Nevertheless, the nematodes collected from C. gariepinus in the Vaal River system are considered S. petterae. This study expands the geographical distribution and adds additional morphological and genetic information for S. petterae, contributing to the limited knowledge of African species of Spinitectus.

瓦尔河系统中来自Clarias gariepus (Siluriformes: Clariidae)的petterae(线虫目:横纹肌目)的附加数据:保守的形态还是高种内遗传变异性?
1884年在非洲南部记录到两种刺虫,分别是1961年的spitectus polli Campana-Rouget和1993年的spitectus petterae Boomker,均来自林波波河水系。北非鲶鱼Clarias gariepinus (Burchell)中发现有棘虫,而棘虫则感染了squeakers, Synodontis spp.。在瓦尔河水系(Orange河流域)的寄生虫学调查中,在cariepinus中采集到了棘虫标本。这些系统相邻但不相连。因此,本研究旨在利用形态学和分子技术对采集的标本进行鉴定。形态学研究包括整个标本和切除的针状体的光镜和扫描电镜。采用18S rDNA、28S rDNA和cox1 mtDNA对标本进行遗传表征。此外,在模式分布点附近还采集了未成熟的petterae标本。形态特征与该属的petterae最相似,而遗传数据与该属的所有现有数据不同。本研究还发现了石竹的其他形态特征,即左右针状结构和假唇上的多孔结构。瓦尔河系的标本与最初被描述为S. petterae的标本不同之处是,第三环后面有额外的刺,缺乏尾鳍,全身和雄性食道长度可变。基于18S rDNA,模式地的单倍型与研究材料的差异很小,支持形态学鉴定。然而,28S rDNA和更明显的cox1 mtDNA在这些地区的标本之间显示出很大的差异,需要进一步研究。本研究中产生的单倍型与坦桑尼亚和埃及的S. petterae的特征高度不同。然而,从瓦尔河系统中采集的C. gariepinus的线虫被认为是S. petterae。本研究扩大了棘虫的地理分布,增加了棘虫的形态和遗传信息,有助于对棘虫非洲种的有限认识。
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来源期刊
Folia Parasitologica
Folia Parasitologica 医学-寄生虫学
CiteScore
2.70
自引率
0.00%
发文量
25
审稿时长
>12 weeks
期刊介绍: FOLIA PARASITOLOGICA, issued in online versions, is an international journal that covers the whole field of general, systematic, ecological and experimental parasitology. It publishes original research papers, research notes and review articles. Contributions from all branches of animal parasitology, such as morphology, taxonomy, biology, biochemistry, physiology, immunology, molecular biology and evolution of parasites, and host-parasite relationships, are eligible. Novelty and importance in the international (not local or regional) context are required. New geographical records of parasites, records of new hosts, regional parasite and/or host surveys (if they constitute the principal substance of manuscript), local/regional prevalence surveys of diseases, local/regional studies on epidemiology of well known diseases and of parasite impact on human/animal health, case reports, routine clinical studies and testing of established diagnostic or treatment procedures, will not be considered. One species description will also not be considered unless they include more general information, such as new diagnostic characters, host-parasite associations, phylogenetic implications, etc. Manuscripts found suitable on submission will be reviewed by at least two reviewers.
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