Factors affecting the features of platelet-rich plasma in patients with knee osteoarthritis.

IF 1.1 4区医学 Q3 ORTHOPEDICS

Acta orthopaedica et traumatologica turcica Pub Date : 2023-07-01 DOI:10.5152/j.aott.2023.22077

Sezen Karaborklu Argut, Derya Celik, Omer Naci Naci Ergin, Onder Ismet Kilicoglu

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Abstract

Objective: The aim of this study was to present an analysis of platelet-rich plasma obtained from patients with knee osteoarthritis and reveal the factors affecting its features.

Methods: A total of 62 patients (mean age: 56.68 ± 7.13 years) with symptomatic knee osteoarthritis were included in this study. Age (years), gender, height (m), weight (kg), body mass index (kg/m2), duration of symptoms, smoking status, smoking index, general health status, and physical activity scores were recorded. Whole blood and platelet-rich plasma cell counts were performed with a hematology analyzer. White blood cell, red blood cell, and platelet counts were recorded. According to the dose of injected platelets, efficiency of the procedure, purity of platelet-rich plasma, and activation classification, dose of platelets, efficiency of the procedure (platelet recovery rate, %), and purity of the obtained platelet-rich plasma product (relative composition in platelets, %) were calculated. Correlation analysis between the features of platelet-rich plasma and the patient-related variables, including age, gender, body mass index, smoking status, smoking index, presence of other health conditions, physical activity scores, duration of symptoms, and pain levels, was performed.

Results: Dose of injected platelets, efficiency of the procedure, purity of platelet-rich plasma, and activation analysis showed that the dose of injected platelets was 3.25 billion, the efficiency of the process was 77%, and the purity rate of the platelet-rich plasma was 98.4%. Platelet-rich plasma platelet count was correlated with whole blood platelet count (r = 0.81, P < .001), whole blood white blood cell count (r = 0.39, P = .002), smoking status (r = 0.56, P = .03), smoking index (r = -0.63, P = .002), and the presence of hypertension (r = -0.31, P=.04). Platelet-rich plasma white blood cell and purity of platelet-rich plasma were correlated with the smoking status of the patients (r = 0.52, P = .01; r = 0.64, P = .003, respectively).

Conclusion: This study has demonstrated that high dose and very pure platelet-rich plasma with medium efficiency was yielded with this platelet-rich plasma preparation procedure; whole blood platelet count, the presence of hypertension, and the smoking status of patients affect the features of the obtained platelet-rich plasma.

Level of evidence: Level IV, Diagnostic Study.

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影响膝骨关节炎患者富血小板血浆特征的因素。

目的：本研究的目的是分析膝骨关节炎患者的富血小板血浆，并揭示影响其特征的因素。方法：本研究共纳入62例有症状的膝关节骨性关节炎患者（平均年龄：56.68±7.13岁）。记录年龄（年）、性别、身高（m）、体重（kg）、体重指数（kg/m2）、症状持续时间、吸烟状况、吸烟指数、总体健康状况和体育活动得分。用血液学分析仪进行全血和富含血小板的浆细胞计数。记录白细胞、红细胞和血小板计数。根据注射的血小板的剂量，计算程序的效率、富含血小板的血浆的纯度和活化分类、血小板的剂量、程序的效率（血小板回收率，%）和获得的富含血小板的等离子体产物的纯度（血小板中的相对组分，%）。对富含血小板血浆的特征与患者相关变量（包括年龄、性别、体重指数、吸烟状况、吸烟指数、是否存在其他健康状况、体力活动评分、症状持续时间和疼痛水平）之间的相关性分析。结果：注射血小板的剂量、程序的效率、富血小板血浆的纯度和活化分析显示，注射血小板的量为32.5亿，过程的效率为77%，富血小板血浆纯度为98.4%。富血小板血浆血小板计数与全血血小板计数相关（r=0.81，P<.001），全血白细胞计数（r=0.39，P=.002），吸烟状况（r=0.56，P=.03），吸烟指数（r=-0.63，P=0.002），和高血压的存在（r=-0.31，P=.04）。富含血小板的血浆白细胞和富含血小板血浆的纯度与患者的吸烟状态相关（分别为r=0.52，P=.01；r=0.64，P=.003）准备程序；全血血小板计数、高血压的存在以及患者的吸烟状态影响所获得的富含血小板的血浆的特征。证据级别：四级，诊断性研究。

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来源期刊

Acta orthopaedica et traumatologica turcica ORTHOPEDICS-

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Acta Orthopaedica et Traumatologica Turcica (AOTT) is an international, scientific, open access periodical published in accordance with independent, unbiased, and double-blinded peer-review principles. The journal is the official publication of the Turkish Association of Orthopaedics and Traumatology, and Turkish Society of Orthopaedics and Traumatology. It is published bimonthly in January, March, May, July, September, and November. The publication language of the journal is English. The aim of the journal is to publish original studies of the highest scientific and clinical value in orthopedics, traumatology, and related disciplines. The scope of the journal includes but not limited to diagnostic, treatment, and prevention methods related to orthopedics and traumatology. Acta Orthopaedica et Traumatologica Turcica publishes clinical and basic research articles, case reports, personal clinical and technical notes, systematic reviews and meta-analyses and letters to the Editor. Proceedings of scientific meetings are also considered for publication. The target audience of the journal includes healthcare professionals, physicians, and researchers who are interested or working in orthopedics and traumatology field, and related disciplines.