Regional differences of the sclera in the ocular hypertensive rat model induced by circumlimbal suture.

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2023-01-04 DOI:10.1186/s40662-022-00319-w

Mingfang Xia, Endong Zhang, Fei Yao, Zhaohua Xia, Mingmin Zhou, Xufang Ran, Xiaobo Xia

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Abstract

Purpose: To describe the regional differences of the sclera in ocular hypertension (OHT) models with the inappropriate extension of the ocular axis.

Methods: To discover the regional differences of the sclera at the early stage, OHT models were established using circumlimbal suture (CS) or sclerosant injection (SI). Axial length (AL) was measured by ultrasound and magnetic resonance imaging. The glaucoma-associated distinction was determined by intraocular pressure (IOP) and retrograde tracing of retinal ganglion cells (RGCs). The central thickness of the ganglion cell complex (GCC) was measured by optical coherence tomography. RGCs and collagen fibrils were detected using a transmission electron microscope, furthermore, anti-alpha smooth muscle actin (αSMA) was determined in the early stage after the operation.

Results: Compared with the control group, the eyes in OHT models showed an increased IOP (P < 0.001 in the CS group, P = 0.001 in the SI group), growing AL (P = 0.026 in the CS group, P = 0.043 in the SI group), reduction of central RGCs (P < 0.001 in the CS group, P = 0.017 in the SI group), thinning central GCC (P < 0.001 in the CS group), and a distinctive expression of αSMA in the central sclera in the early 4-week stage after the operation (P = 0.002 in the CS group). Compared with the SI group, the eye in the CS group showed a significantly increased AL (7.1 ± 0.4 mm, P = 0.031), reduction of central RGCs (2121.1 ± 87.2 cells/mm², P = 0.001), thinning central GCC (71.4 ± 0.8 pixels, P = 0.015), and a distinctive expression of αSMA (P = 0.005). Additionally, ultrastructural changes in RGCs, scleral collagen fibers, and collagen crimp were observed in the different regions. Increased collagen volume fraction in the posterior segment of the eyeball wall (30.2 ± 3.1%, P = 0.022) was observed by MASSON staining in the CS group.

Conclusion: Regional differences of the sclera in the ocular hypertensive rat model induced by CS may provide a reference for further treatment of scleral-related eye disorders.

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角膜缘缝合大鼠眼压模型巩膜的区域差异。

目的:探讨高眼压(OHT)模型眼轴不适当伸展时巩膜的区域差异。方法:采用环缘缝合(CS)和硬化剂注射(SI)两种方法建立OHT模型，观察早期巩膜的区域差异。通过超声和磁共振成像测量轴向长度(AL)。青光眼相关的区分是通过眼内压(IOP)和视网膜神经节细胞(RGCs)的逆行示踪来确定的。采用光学相干断层成像技术测量神经节细胞复合体(GCC)的中心厚度。透射电镜检测RGCs和胶原原纤维，术后早期检测抗α -平滑肌肌动蛋白(αSMA)水平。结果:与对照组比较，OHT模型眼IOP升高(P 2, P = 0.001)，中央GCC变薄(71.4±0.8像素，P = 0.015)， αSMA明显表达(P = 0.005)。不同区域RGCs、巩膜胶原纤维和胶原卷曲的超微结构变化。CS组眼球壁后段胶原体积分数升高(30.2±3.1%，P = 0.022)。结论:CS致高眼压模型大鼠巩膜的区域差异可为进一步治疗巩膜相关性眼病提供参考。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.