Analysis of Taurine in Infant Formulas and Adult Nutritionals by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry: First Action 2022.03.

IF 1.7 4区 农林科学 Q3 CHEMISTRY, ANALYTICAL
Brendon D Gill, Jackie E Wood
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引用次数: 0

Abstract

Background: Taurine is recognized as an essential growth factor and as being critical in the maintenance of functional tissue regulation.

Objective: To evaluate the analytical performance of a hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method for compliance with AOAC Standard Method Performance Requirements (SMPR®) for taurine analysis described in SMPR 2014.013.

Method: Following protein precipitation with Carrez solutions, taurine is extracted and separated by HILIC with detection by triple quadrupole MS using multiple reaction monitoring (MRM). Stable isotope labeled (SIL) taurine internal standard is used for quantification to correct for losses in extraction and variations in ionization in the ion source.

Results: The method was shown to meet the requirements specified in the SMPR with a linear range of 0.27-2700 mg/hg RTF (ready-to-feed), a limit of detection of 0.14 mg/hg RTF, acceptable recovery of 97.2-100.1%, and acceptable repeatability of 1.6-6.4% relative standard deviation. Additionally, the method was found to have no statistically significant bias compared with reference values for National Institute of Standards and Technology (NIST) 1849a certified reference material (CRM) (P-value = 0.95) and 1869 CRM (P-value = 0.31), and with results from AOAC 997.05 (P-value = 0.10).

Conclusions: A recent review of the method and validation data by the Stakeholder Program on Infant Formula and Adult Nutritionals (SPIFAN) Expert Review Panel (ERP) found that this method met all the criteria for analysis of taurine specified in SMPR 2014.013 and voted to adopt this method as First Action AOAC Official MethodSM2022.03.

Highlights: A method for the analysis of taurine in infant formulas and adult nutritionals by HILIC-MS/MS is described. A single-laboratory validation (SLV) study demonstrated the applicability of the method to meet requirements of SMPR 2014.013. In December 2022, the SPIFAN ERP voted to adopt this method as First Action AOAC Official Method 2022.03.

亲水相互作用液相色谱-质谱法分析婴儿配方奶粉和成人营养品中的牛磺酸:第一步2022.03。
背景:牛磺酸被认为是一种重要的生长因子,在维持功能性组织调节中至关重要。目的:评估亲水相互作用液相色谱-串联质谱法(HILIC-MS/MS)的分析性能,以符合SMPR 2014.013中描述的AOAC牛磺酸分析标准方法性能要求(SMPR®)。方法:用Carrez溶液沉淀蛋白质后,牛磺酸通过HILIC提取和分离,并通过多重反应监测(MRM)的三重四极杆MS检测。稳定同位素标记(SIL)牛磺酸内标用于定量,以校正离子源中提取的损失和电离的变化。结果:该方法符合SMPR中规定的要求,线性范围为0.27-2700 mg/hg RTF(即食),检测限为0.14 mg/hg RTF,可接受回收率为97.2-100.1%,可接受重复性为1.6-6.4%的相对标准偏差。此外,与美国国家标准与技术研究所(NIST)1849a认证参考材料(CRM)的参考值(P值 = 0.95)和1869 CRM(P值 = 0.31),以及AOAC 997.05的结果(P值 = 0.10)。结论:婴儿配方奶粉和成人营养品利益相关者计划(SPIFAN)专家审查小组(ERP)最近对该方法和验证数据进行的审查发现,该方法符合SMPR 2014.013中规定的牛磺酸分析的所有标准,并投票将该方法作为AOAC官方方法SM2022.03的第一行动通过HILIC-MS/MS描述了婴儿配方奶粉和成人营养品。一项单实验室验证(SLV)研究证明了该方法的适用性,以满足SMPR 2014.013的要求。2022年12月,SPIFAN ERP投票决定采用该方法作为AOAC官方第一行动方法 2022.03。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of AOAC International
Journal of AOAC International 医学-分析化学
CiteScore
3.10
自引率
12.50%
发文量
144
审稿时长
2.7 months
期刊介绍: The Journal of AOAC INTERNATIONAL publishes the latest in basic and applied research in analytical sciences related to foods, drugs, agriculture, the environment, and more. The Journal is the method researchers'' forum for exchanging information and keeping informed of new technology and techniques pertinent to regulatory agencies and regulated industries.
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