Cutaneous Photorejuvenation of Light Emitting Diodes via the Melatonin Membrane Receptor Pathway.

IF 1.5 4区医学 Q3 DERMATOLOGY

Annals of Dermatology Pub Date : 2022-12-01 DOI:10.5021/ad.21.092

Soo Hyeon Bae, Jun Hyeong Park, Soo-Hyun Kim, Sook Jung Yun, Jae Gwan Kim, Jee-Bum Lee

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引用次数: 0

Abstract

Background: Melatonin receptors are present in the human skin and retina. These receptors can be stimulated by light emitting diodes (LEDs) at specific wavelengths, thereby inducing cutaneous photorejuvenation. However, the underlying mechanism remains unclear.

Objective: To evaluate the influence of LEDs at specific wavelengths on melatonin membrane receptor (MT1) and cutaneous photorejuvenation via the MT1 pathway in vitro.

Methods: Normal human dermal fibroblasts (HDFs) were irradiated using LEDs at different wavelengths (410~940 nm) at a dose of 1 J/cm². MT1 activity was evaluated after melatonin stimulation and LED irradiation. Thereafter, the expressions of collagen (COL) and matrix metalloproteinases (MMPs), with and without luzindole (MT1/2 receptor antagonist), were investigated via semi-quantitative reverse transcription polymerase chain reaction (PCR), real-time PCR, western blotting, and enzyme-linked immunosorbent assay.

Results: In HDFs, the MT1 mRNA and protein levels increased significantly in response to melatonin (dose, 50 nM) (p<0.01) and LED irradiation at 595, 630, 850, and 940 nm (p<0.01). LED irradiation up-regulated COL type I and down-regulated MMP-1. Compared to LED irradiation without luzindole, LED irradiation with luzindole produced no significant increase in COL type I mRNA and protein levels (p<0.01).

Conclusion: We found that LED irradiation induces collagen synthesis and MMP-1 inhibition in HDFs via MT1 activation. Additionally, multiple LED wavelengths (595, 630, 850, and 940 nm) stimulated MT1 in HDFs, unlike in the eyes, where only blue light induced plasma melatonin suppression. This suggests the possibility of the melatoninergic pathway in photorejuvenation.

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通过褪黑素膜受体途径的发光二极管皮肤光再生。

背景:褪黑激素受体存在于人类皮肤和视网膜中。这些受体可以被发光二极管(led)在特定波长下刺激，从而诱导皮肤光再生。然而，其潜在机制尚不清楚。目的:探讨特定波长led对体外褪黑素膜受体(MT1)及MT1通路皮肤光嫩化的影响。方法:用不同波长(410~940 nm)的led照射正常人真皮成纤维细胞(HDFs)，照射剂量为1 J/cm²。褪黑素刺激和LED照射后评估MT1活性。随后，采用半定量反转录聚合酶链反应(PCR)、实时荧光定量PCR、western blotting和酶联免疫吸附法检测添加和不添加luzindole (MT1/2受体拮抗剂)时胶原蛋白(COL)和基质金属蛋白酶(MMPs)的表达。结果:在HDFs中，褪黑素(剂量，50 nM)显著增加了MT1 mRNA和蛋白水平(ppp)。结论:我们发现LED照射通过MT1激活诱导HDFs中胶原合成和MMP-1抑制。此外，多种LED波长(595、630、850和940 nm)刺激HDFs中的MT1，不像在眼睛中，只有蓝光诱导血浆褪黑激素抑制。这提示了褪黑激素通路在光嫩化中的可能性。

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来源期刊

Annals of Dermatology 医学-皮肤病学

CiteScore

1.60

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Annals of Dermatology (Ann Dermatol) is the official peer-reviewed publication of the Korean Dermatological Association and the Korean Society for Investigative Dermatology. Since 1989, Ann Dermatol has contributed as a platform for communicating the latest research outcome and recent trend of dermatology in Korea and all over the world. Ann Dermatol seeks for ameliorated understanding of skin and skin-related disease for clinicians and researchers. Ann Dermatol deals with diverse skin-related topics from laboratory investigations to clinical outcomes and invites review articles, original articles, case reports, brief reports and items of correspondence. Ann Dermatol is interested in contributions from all countries in which good and advanced research is carried out. Ann Dermatol willingly recruits well-organized and significant manuscripts with proper scope throughout the world.