The Protective Effect of Trichosanthes kirilowii Peel Polysaccharide on the Oxidative Damaged HepG2 and HUASMC Cells.

IF 1.4 4区生物学 Q4 GENETICS & HEREDITY

Genetics research Pub Date : 2022-01-01 DOI:10.1155/2022/1792977

Jinli Zhang, Heren Gao, Liya Zhu, Xiangyu Yuan, Xi Yang, Min Xu, Yang Yang

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引用次数: 1

Abstract

Background: Oxidative stress is an important cause of liver disease and atherosclerosis. Natural substances with antioxidant activity are good drugs for treating liver disease and atherosclerosis. Trichosanthes kirilowii Peel Polysaccharide (TKPP) can remove DPPH (2,2-Diphenyl-1-picrylhydrazyl) free radicals and hydroxyl free radicals in vitro, which shows antioxidant activity. Therefore, it is speculated that it can protect human hepatoma cell line (HepG2) and umbilical artery smooth muscle cell (HUASMC) against oxidative damage by hydrogen peroxide (H₂O₂).

Methods: Oxidative damage cell models of HepG2 and HUASMC were induced by H₂O₂. HepG2 and HUASMC were divided into blank group, H₂O₂ injury group, TKPP treatment group, and glutathione (GSH) positive control group. Cell Counting Kit-8 (CCK-8) was used to detect cell viability. The level of total GSH and the amount of Nitric oxide (NO) secreted by cells were detected by specific kits. The gene and protein expressions of catalase (CAT) and superoxide dismutase (SOD) were detected by fluorescence quantitative PCR and Western Blot.

Results: In these two kinds of cells, compared with the control group, the survival rate, total GSH level, and NO secretion, CAT and SOD gene and protein expressions were significantly decreased in the H₂O₂ damaged group. In the TKPP treatment group, the cell survival rate was significantly elevated with the increase of the polysaccharide concentration, and the total GSH level, NO secretion, CAT and SOD gene expression, and protein expression levels were also significantly increased.

Conclusion: TKPP can improve the activities of HepG2 and HUASMC cells damaged by H₂O₂ and protect the cellular antioxidant system.

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栝楼皮多糖对氧化损伤的HepG2和HUASMC细胞的保护作用。

背景:氧化应激是肝脏疾病和动脉粥样硬化的重要原因。具有抗氧化活性的天然物质是治疗肝脏疾病和动脉粥样硬化的良好药物。天花粉果皮多糖(TKPP)在体外具有清除DPPH(2,2-二苯基-1-苦酰肼基)自由基和羟基自由基的作用，具有较强的抗氧化活性。因此，推测其可保护人肝癌细胞系(HepG2)和脐动脉平滑肌细胞(HUASMC)免受过氧化氢(H2O2)的氧化损伤。方法:采用H2O2诱导HepG2和HUASMC氧化损伤细胞模型。将HepG2和HUASMC分为空白组、H2O2损伤组、TKPP治疗组和谷胱甘肽(GSH)阳性对照组。细胞计数试剂盒-8 (CCK-8)检测细胞活力。用特异性试剂盒检测细胞总谷胱甘肽水平和一氧化氮(NO)分泌量。采用荧光定量PCR和Western Blot检测过氧化氢酶(CAT)和超氧化物歧化酶(SOD)基因和蛋白的表达。结果:与对照组相比，H2O2损伤组两种细胞的存活率、总GSH水平、NO分泌、CAT、SOD基因及蛋白表达均显著降低。TKPP处理组细胞存活率随多糖浓度的升高而显著升高，总GSH水平、NO分泌、CAT、SOD基因表达及蛋白表达水平均显著升高。结论:TKPP能提高H2O2损伤的HepG2和HUASMC细胞的活性，保护细胞抗氧化系统。

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来源期刊

Genetics research 生物-遗传学

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Genetics Research is a key forum for original research on all aspects of human and animal genetics, reporting key findings on genomes, genes, mutations and molecular interactions, extending out to developmental, evolutionary, and population genetics as well as ethical, legal and social aspects. Our aim is to lead to a better understanding of genetic processes in health and disease. The journal focuses on the use of new technologies, such as next generation sequencing together with bioinformatics analysis, to produce increasingly detailed views of how genes function in tissues and how these genes perform, individually or collectively, in normal development and disease aetiology. The journal publishes original work, review articles, short papers, computational studies, and novel methods and techniques in research covering humans and well-established genetic organisms. Key subject areas include medical genetics, genomics, human evolutionary and population genetics, bioinformatics, genetics of complex traits, molecular and developmental genetics, Evo-Devo, quantitative and statistical genetics, behavioural genetics and environmental genetics. The breadth and quality of research make the journal an invaluable resource for medical geneticists, molecular biologists, bioinformaticians and researchers involved in genetic basis of diseases, evolutionary and developmental studies.