Clinical and laboratory findings following transplantation of allogeneic adipose-derived mesenchymal stromal cells in knee osteoarthritis, a brief report.

IF 2.8 4区医学 Q3 CELL BIOLOGY

Connective Tissue Research Pub Date : 2022-11-01 DOI:10.1080/03008207.2022.2074841

Bahareh Sadri, Atena Tamimi, Shirin Nouraein, Abolfazl Bagheri Fard, Javad Mohammadi, Mehdi Mohammadpour, Mohammad Hassanzadeh, Amir Bajouri, Hoda Madani, Maryam Barekat, Shahedeh Karimi Torshizi, Mahrooz Malek, Maede Ghorbani Liastani, Alireza Beheshti Maal, Maryam Niknejadi, Massoud Vosough

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引用次数: 6

Abstract

Background: Mesenchymal stromal cells (MSCs) injection has been proposed as an innovative treatment for knee osteoarthritis (KOA). Since, allogeneic MSCs can be available as off-the-shelf products, they are preferable in regenerative medicine. Among different sources for MSCs, adipose-derived MSCs (AD-MSCs) appear to be more available.

Methods: Three patients with KOA were enrolled in this study. A total number of 100 × 10⁶ AD-MSCs was injected intra-articularly, per affected knee. They were followed up for 6 months by the assessment of clinical outcomes, magnetic resonance imaging (MRI), and serum inflammatory biomarkers.

Results: The primary outcome of this study was safety and feasibility of allogeneic AD-MSCs injection during the 6 months follow-up. Fortunately, no serious adverse events (SAEs) were reported. Assessment of secondary outcomes of visual analogue scale (VAS), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and knee osteoarthritis outcome score (KOOS) indicated improvement in all patients. Comparison between baseline and endpoint findings of MRI demonstrated a slight improvement in two patients. In addition, decrease in serum cartilage oligomeric matrix protein (COMP) and hyaluronic acid (HA) indicated the possibility of reduced cartilage degeneration. Moreover, quantification of serum interleukin-10 (IL-10) and interleukin-6 (IL-6) levels indicated that the host immune system immunomodulated after infusion of AD-MSCs.

Conclusion: Intra-articular injection of AD-MSCs is safe and could be effective in cartilage regeneration in KOA. Preliminary assessment after six-month follow-up suggests the potential efficacy of this intervention which would need to be confirmed in randomized controlled trials on a larger population.

Trial registration: This study was registered in the Iranian registry of clinical trials (https://en.irct.ir/trial/46) in 24 April 2018 with identifier IRCT20080728001031N23.

查看原文本刊更多论文

临床和实验室结果移植异体脂肪来源间充质细胞在膝关节骨关节炎，一个简短的报告。

背景:间充质基质细胞(MSCs)注射被认为是治疗膝骨关节炎(KOA)的一种创新方法。由于同种异体间充质干细胞可以作为现成的产品，它们在再生医学中更受欢迎。在不同来源的间充质干细胞中，脂肪来源的间充质干细胞(AD-MSCs)似乎更容易获得。方法:选取3例KOA患者作为研究对象。每个患膝关节内注射100 × 106个AD-MSCs。通过评估临床结果、磁共振成像(MRI)和血清炎症生物标志物，对他们进行了6个月的随访。结果:本研究的主要结果是在6个月的随访期间注射同种异体AD-MSCs的安全性和可行性。幸运的是，没有严重不良事件(SAEs)的报道。通过视觉模拟量表(VAS)、西安大略大学和麦克马斯特大学骨关节炎指数(WOMAC)和膝关节骨关节炎结局评分(oos)评估，所有患者均有改善。MRI的基线和终点结果比较显示两名患者有轻微改善。此外，血清软骨寡聚基质蛋白(COMP)和透明质酸(HA)的降低表明软骨退变可能减少。此外，血清白细胞介素-10 (IL-10)和白细胞介素-6 (IL-6)水平的定量分析表明，AD-MSCs输注后，宿主免疫系统具有免疫调节作用。结论:关节内注射AD-MSCs对KOA软骨再生是安全有效的。6个月随访后的初步评估表明，这种干预措施的潜在疗效需要在更大人群的随机对照试验中得到证实。试验注册:本研究于2018年4月24日在伊朗临床试验注册中心(https://en.irct.ir/trial/46)注册，标识符为IRCT20080728001031N23。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Connective Tissue Research 生物-细胞生物学

CiteScore

6.60

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： The aim of Connective Tissue Research is to present original and significant research in all basic areas of connective tissue and matrix biology. The journal also provides topical reviews and, on occasion, the proceedings of conferences in areas of special interest at which original work is presented. The journal supports an interdisciplinary approach; we present a variety of perspectives from different disciplines, including Biochemistry Cell and Molecular Biology Immunology Structural Biology Biophysics Biomechanics Regenerative Medicine The interests of the Editorial Board are to understand, mechanistically, the structure-function relationships in connective tissue extracellular matrix, and its associated cells, through interpretation of sophisticated experimentation using state-of-the-art technologies that include molecular genetics, imaging, immunology, biomechanics and tissue engineering.