Application of Immunohistochemistry in Cytology.

IF 1.3 4区 医学 Q3 ANATOMY & MORPHOLOGY
Yan Shi, Melissa Yee-Chang, Shan-Rong Shi
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引用次数: 0

Abstract

Immunohistochemistry (IHC), also referred to as immunocytochemistry in cytology literature, has revolutionized the practice of cytopathology. Because of the complexity of cytology preparation and limited diagnostic material, performing IHC remains a challenge. Formalin-fixed paraffin-embedded (FFPE) cell block (CB) is the optimal choice for IHC. In this review, the approaches for improving CB preparation will be discussed. When CB material is not available, various cytology specimens can also be used for IHC. With the utilization of Antigen Retrieval (AR) technique, these nonformalin-fixed cytology specimens can achieve successful IHC staining, comparable with the results from FFPE tissue sections. In the last part of this review, we will discuss the use of positive controls and the important role of AR in standardization of IHC in cytology.

免疫组织化学在细胞学中的应用。
免疫组织化学(IHC),在细胞学文献中也被称为免疫细胞化学,已经彻底改变了细胞病理学的实践。由于细胞学准备的复杂性和有限的诊断材料,进行免疫组化仍然是一个挑战。甲醛固定石蜡包埋(FFPE)细胞块(CB)是免疫组化的最佳选择。本文将讨论改进炭黑制备的途径。当没有CB材料时,各种细胞学标本也可用于免疫组化。利用抗原检索(AR)技术,这些非福尔马林固定细胞学标本可以成功地进行免疫组化染色,与FFPE组织切片的结果相当。在这篇综述的最后一部分,我们将讨论阳性对照的使用和AR在细胞学IHC标准化中的重要作用。
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来源期刊
Applied Immunohistochemistry & Molecular Morphology
Applied Immunohistochemistry & Molecular Morphology ANATOMY & MORPHOLOGY-MEDICAL LABORATORY TECHNOLOGY
CiteScore
3.20
自引率
0.00%
发文量
153
期刊介绍: ​Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist. Official Journal of the International Society for Immunohistochemisty and Molecular Morphology​.
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